Single-nucleus RNA-sequencing (snRNA-seq) is emerging as a promising solution to scRNA-seq due to its reduced ionized transcription bias and compatibility with richer samples. This approach will provide an exciting opportunity for in-depth exploration of billions of formalin-fix...
Here, we perform single-nucleus RNA-sequencing (snRNA-seq) on nearly 300,000 nuclei from the parietal cortex of AD autosomal dominant (APP and PSEN1) and risk-modifying variant (APOE, TREM2 and MS4A) carriers. Within individual cell types, we capture genes commonly dysregulated across variant...
Our goal was to use single nucleus RNA sequencing to find transcriptional similarities between late-onset myopathy patients. To this end, we developed a novel nuclei isolation approach from frozen human skeletal muscle biopsies. We isolated nuclei from the muscle of 10 patients with a diverse range...
#科研# 单细胞核RNA测序(single-nucleus RNA sequencing, snRNA-seq)[思考];10x Genomics和BD Rhapsody单细胞测序平台[思考] http://t.cn/A6UNpPAs
Here, we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional diversity within multinucleated skeletal myofibers. Nuclei from mouse skeletal muscle were profiled across the lifespan, which revealed the emergence of distinct myonuclear populations in postnatal ...
Defining cellular and molecular identities within the kidney is necessary to understand its organization and function in health and disease. Here we demonstrate a reproducible method with minimal artifacts for single-nucleus Droplet-based RNA sequencing
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
Single cell RNA-sequencing (scRNASeq) has advanced our understanding of lung biology, but utility is limited by the need for fresh samples, loss of cell types by death or inadequate dissociation, and transcriptional stress responses induced during tissue digestion. Single nucleus RNASeq (snRNASeq)...
To reveal the impact of chromatin accessibility on gene expression, we integrated sNucRNA-seq and sNucATAC-seq data and demonstrated that cell-type-specific marker genes display cell-type-specific patterns of chromatin accessibility. Our data suggest that the differential chromatin accessibility is a ...
First, we used single-nucleus RNA sequencing to estimate RNA velocity. Compared to single-cell sequencing, snRNA-seq is more amenable to transcriptomic profiling of postmortem samples because isolated nuclei are intact in frozen tissues29. Moreover, dissociating whole cells from the brain is ...