Here, we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional diversity within multinucleated skeletal myofibers. Nuclei from mouse skeletal muscle were profiled across the lifespan, which revealed the emergence of distinct myonuclear populations in postnatal ...
RNA sequencing was carried out using the Illumina NovaSeq System. Data processing and core analysis of snRNA-seq dataset The sequencing data were processed using the zUMIs pipeline (version 2.3.0)9. The pipeline was used to first discard reads with low-quality barcodes and UMIs, and then to ...
Cells, as the fundamental units of life, contain multidimensional spatiotemporal information. Single-cell RNA sequencing (scRNA-seq) is revolutionizing biomedical science by analyzing cellular state and intercellular heterogeneity. Undoubtedly, single-cell transcriptomics has emerged as one of the most vibra...
Both the single-nucleus (snRNA-seq) and single-cell (scRNA-seq) RNA-sequencing datasets were produced using cultured human islet samples obtained from the same donor. In particular, we generated single-cell and single-nucleus libraries using 4 technical replicates for each method. Gene counts were...
Single-nucleus RNA-sequencing (snRNA-seq) has emerged as a powerful approach to interrogating the underlying transcriptional landscape of the cellularly-complex human brain. SnRNA-seq has been used to study AD in different cohorts and brain regions, including the entorhinal cortex15, temporal neocorte...
Single-cell RNA sequencing (ScRNA-seq), single-nucleus RNA sequencing (SnRNA-seq), and Xenium-based spatial transcriptomics analyses were employed to evaluate the changes in mRNA expression in glial cells and neurons. Results From the ScRNA-seq and SnRNA-seq data, 31,390 glial cells and 48,...
This paper describes a new approach for spatial single-nucleus genomic profiling in frozen tissue sections using spatial barcoding, which can be applied to multiple modalities including snRNA-seq, ATAC-seq and T cell antigen receptor sequencing. This tool can be applied for multimodal, orthogonal ...
In plants, scRNA-seq experiments have been conducted using protoplasts and nuclei derived from roots, leaves, shoot apical meristems, stems, and inflorescences of various species. scRNA-seq using nuclei samples is also called single-nucleus RNA sequencing (snRNA-seq). Nevertheless, most studies hav...
Recent advances in single-nucleus RNA sequencing (snRNA-seq) have provided an unprecedented ability to disentangle cellular-level transcriptomic alterations and heterogeneity in Alzheimer’s disease (AD)1,2. Early differential expression with neuropathological AD progression have been found to be cell-...
Single-nucleus RNA sequencing Single-nucleus dissociation of the mouse spinal cord was performed as previously described27,51. Animals were first euthanized by isoflurane inhalation and cervical dislocation. The lumbar spinal cord site was rapidly dissected and frozen on dry ice. Spinal cords were dou...