为了验证鉴定小鼠interneurons的Dlx1/2调控网络,作者分析了人脑的sNuc-Seq(Single nuclei RNA-Seq)数据集。 在人类脑sNuc-Seq数据集上,SCENIC也鉴定出由DLX1/2强烈驱动的interneurons细胞群,该群具有与小鼠相同的motif,并且识别出一组保守的靶标,包括DLX1本身。 接下来,作者将这种跨物种分析扩展到其他细胞类型。与基...
Schematic overview of the GEM-X technology workflow. A typical workflow starts with user-supplied cells or nuclei that are combined with our GEM-X reagents and consumables, enabling partitioning, cell lysis, and barcoding of cellular information. These barcoded transcripts are then amplified and conv...
Single-cell RNA sequencing methods Several methods, full-length or tag-based, were published under scRNA-seq where each protocol offers its own advantages and applicability. Generally, scRNA-seq includes four steps: (a) single cells or single nuclei isolation and lysis from extracellular matrix and...
采用 DropSeq,DroNc-seq和10xChromium平台(平台差异详见Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems)分别进行单细胞和单细胞核转录组分析,比较不同的样品类型对单细胞结果产生的影响。
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
Single-cell/nuclei RNA sequencing (sc/snRNA-seq) is widely used for profiling cell-type gene expression in brain research. An important but frequently underappreciated issue is the data quality in terms of precision and accuracy. We evaluated precision using data from 14 human brain studies with ...
Single-Cell Sequencing Services Single-Cell RNA-Seq Analyze transcriptome heterogeneity at the single-cell level. GENEWIZ from Azenta offers optimized workflows for 5’ and 3’ gene expression libraries to uncover cellular differences that are masked by bulk RNA sequencing. ...
1C. Across all cell types, we observed a significant reduction in the proportion of oligodendrocytes in the OVT73 derived tissue compared with control cases (ANOVA, P = 1.24×10−5).Figure 1 Single nuclei RNA-seq of the OVT73 sheep striatum. (A) Experimental design. Nuclei were ...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with di...
Here we used a rat model of permanent middle cerebral artery occlusion (MCAO) to investigate acute cell type specific transcriptional perturbations, at single-cell resolution in the acute phase following cerebral ischemic injury (Fig.1a). Fig. 1: snRNAseq reveals differential cell cluster abundance...