为了验证鉴定小鼠interneurons的Dlx1/2调控网络,作者分析了人脑的sNuc-Seq(Single nuclei RNA-Seq)数据集。 在人类脑sNuc-Seq数据集上,SCENIC也鉴定出由DLX1/2强烈驱动的interneurons细胞群,该群具有与小鼠相同的motif,并且识别出一组保守的靶标,包括DLX1本身。 接下来,作者将这种跨物种分析扩展到其他细胞类型。与基...
Schematic overview of the GEM-X technology workflow. A typical workflow starts with user-supplied cells or nuclei that are combined with our GEM-X reagents and consumables, enabling partitioning, cell lysis, and barcoding of cellular information. These barcoded transcripts are then amplified and conv...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with di...
In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings. Watch Now ...
Mapping of single-nuclei data to single-cell clusters A recent publication9generated single-nucleus RNA-seq data from dorsolateral prefrontal cortex in 48 donors (comprising subsets of donors with and without diagnoses of Alzheimer’s disease) from the ROSMAP cohort (data available at Synapse (https...
Single-cell RNA sequencing methods Several methods, full-length or tag-based, were published under scRNA-seq where each protocol offers its own advantages and applicability. Generally, scRNA-seq includes four steps: (a) single cells or single nuclei isolation and lysis from extracellular matrix and...
如今,单细胞RNA测序(scRNA-seq)是一种成熟可靠的方法,使研究人员能够深入研究由细胞异质性驱动的生物复杂性。使用scRNA-seq技术的科学家已经取得了令人难以置信的发现。 2009年scRNA-seq的出现,就像美国宇航局在20世纪90年代发射哈勃太空望远镜一样,让我们对一个被低估的宇宙有了前所未有的认识。从那时起,从植物生物...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
contained in the nucleus of the cell 13 , and it avoids harsh treatment that would perturb gene expression. Through extensive comparisons of nuclear and cellular transcriptomes, we demonstrated that nuclei can substitute for whole cells in most RNA-seq applications ...
在一篇发表于《RAPID COMMUNICATION》的“单细胞核RNA测序比单细胞RNA测序可以发现更多小鼠肾脏纤维细胞稀少的细胞类型和未知的细胞状态”文章中提到,采用 DropSeq,DroNc-seq和10xChromium平台(平台差异详见Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems)分别进行单细胞和单细胞核...