为了验证鉴定小鼠interneurons的Dlx1/2调控网络,作者分析了人脑的sNuc-Seq(Single nuclei RNA-Seq)数据集。 在人类脑sNuc-Seq数据集上,SCENIC也鉴定出由DLX1/2强烈驱动的interneurons细胞群,该群具有与小鼠相同的motif,并且识别出一组保守的靶标,包括DLX1本身。 接下来,作者将这种跨物种分析扩展到其他细胞类型。与基...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with di...
Previous single-cell approaches for RNA-seq from tissues include cell dissociation using protease treatment at 30 °C, which is known to alter the transcriptome. We isolate nuclei at 4 °C from tissue homogenates, which cause minimal damage. Nuclear transcriptomes can be obtained from postmortem ...
采用 DropSeq,DroNc-seq和10xChromium平台(平台差异详见Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems)分别进行单细胞和单细胞核转录组分析,比较不同的样品类型对单细胞结果产生的影响。
然而,利用高度敏感的单核或单细胞RNA-seq,研究人员可以分析活跃分化细胞样本中单个细胞的转录物水平,从而为研究人员提供解析瞬时细胞状态所需的分辨率。识别这些短暂的转变可以帮助我们提高对发育生物学、衰老的理解,并确定最有效的细胞群进行细胞治疗。 Chromium GEM-X provides improved cell recovery efficiency. Cell ...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
如今,单细胞RNA测序(scRNA-seq)是一种成熟可靠的方法,使研究人员能够深入研究由细胞异质性驱动的生物复杂性。使用scRNA-seq技术的科学家已经取得了令人难以置信的发现。 2009年scRNA-seq的出现,就像美国宇航局在20世纪90年代发射哈勃太空望远镜一样,让我们对一个被低估的宇宙有了前所未有的认识。从那时起,从植物生物...
Cells and nuclei were processed using the 10x Genomics platform, and sequencing data analyzed by Seurat. 16,110 single nucleus and 11,934 single cell transcriptomes were generated. Gene detection rates were equivalent in snRNASeq and scRNASeq (~1,700 genes and 3,000 UMI per cell) when ...
PIPseq™ Nuclei Isolation Kit Supplier:Fluent Biosciences FBS-SCR-T100-2-V4.05-FLU PIPseq™ T100 3' Single Cell RNA Kit v4.0PLUS Supplier:Fluent Biosciences FBS-SCR-T100-STKIT-FLU PIPseq™ T100 3' Single Cell Starter Equipment Kit ...
concentration was adjusted to 1200 nuclei/μL and followed immediately by the 10X Genomics® Single Cell Protocol. We generated snuclRNA-seq libraries using the 10X Chromium V(D)J 5` chemistry for 10,000 cells per sample and sequenced 50,000 reads per cell from the 3 frozen human ...