Single-cell ribonucleic acid (RNA) sequencing (scRNA-seq) is an effective technique for estimating the cellular composition and transcriptional profiles of individual cells from fresh tissue. Single-nucleus RNA sequencing (snRNA-seq) is necessary to perform this type of analysis in frozen or ...
Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated from fresh tumors, single-nucleus RNA-Seq (snRNA-Seq) is needed to profile frozen or hard-to-dissociate tumors. Each requires customization to different tissue and tumor types, posing a barrier to adoption. Here, we...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
Single cell mRNA sequencing technologies have transformed our understanding of cellular heterogeneity and identity. For sensitive discovery or clinical marker estimation where high transcript capture per cell is needed only plate-based techniques current
[1,2]. Previous studies used single-cell or single-nucleus RNA sequencing (scRNA-seq or snRNA-seq) to investigate cellular changes or drug responses in diabetic kidneys. Single-nucleus transcriptomics on early human diabetic kidneys suggests increased potassium secretion in the distal nephron and ...
#科研# 单细胞核RNA测序(single-nucleus RNA sequencing, snRNA-seq)[思考];10x Genomics和BD Rhapsody单细胞测序平台[思考] http://t.cn/A6UNpPAs
MS4Amicroglia have dysregulated complement cascade genes, andAPOEε4 inhibitory neurons display signs of ferroptosis. All cell types have enriched states in autosomal dominant carriers. We leverage differential expression and single-nucleus ATAC-seq to map GWAS signals to effector cell types including ...
Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos Qianqi Zhu, Miaoying Tan, Chengniu Wang, Yufei Chen, Chenfei Wang, Junqi Zhang, Yijun Gu, Yuqi Guo, Jianpeng Han, Lei Li, Rongrong Jiang, Xudong Fan, Huimin Xie...
Single-cell RNA-seq (scRNA-seq) enables the quantification of RNA abundance at the resolution of individual cells4. The maturation of single-cell technologies now enables large-scale comparisons of cell states within complex tissues, thus providing the appropriate resolution to dissect cell-type-speci...
Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but archived human brain samples still pose a challenge to current high-throughput platforms. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting diluti...