Cite: Habib N, Li Y, Heidenreich M. et al. Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons.Science. 2016 Aug 26;353(6302):925-8. Abstract 单细胞 RNA 测序提供了有关细胞类型和状态的丰富信息。然而,捕获成体神经元发生等罕见动态过程非常困难,因为从成体组织中分...
Single-nucleus RNA-sequencing (snRNA-seq) is emerging as a promising solution to scRNA-seq due to its reduced ionized transcription bias and compatibility with richer samples. This approach will provide an exciting opportunity for in-depth exploration of billions of formalin-...
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
#科研# 单细胞核RNA测序(single-nucleus RNA sequencing, snRNA-seq)[思考];10x Genomics和BD Rhapsody单细胞测序平台[思考] http://t.cn/A6UNpPAs
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
Preparation of single-nucleus suspensions Nuclei from frozen neural retinal tissue was isolated using RNase-free lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40). The frozen tissue was resuspended in ice-cold lysis buffer and triturated to break the tissue structure....
To characterize the transcriptional profile of myonuclei within myofibers, we developed single nucleus RNA-seq (snRNA-seq) experiments using a droplet-based platform on purified nuclei from different skeletal muscles of adult mice (Fig.1aand Supplementary Fig.1a–d). By using Seurat software25and ...
有一部分细胞核也编码线粒体相关基因。虽然现存生物体中绝大多数作用于线粒体的蛋白质，是由细胞核DNA...
Single cell RNA-sequencing (scRNASeq) has advanced our understanding of lung biology, but utility is limited by the need for fresh samples, loss of cell types by death or inadequate dissociation, and transcriptional stress responses induced during tissue digestion. Single nucleus RNASeq (snRNASeq)...
Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocam... A Regev,E Drokhlyansky,I Avraham-Davidi,... 被...