single-nucleus RNA-seqSingle-nucleus RNA sequencing (snRNA-seq) is used as an alternative to single-cell RNA-seq, as it allows transcriptomic profiling of frozen tissue. However, it is unclear whether snRNA-seq is able to detect cellular state in human tissue. Indeed, snRNA-seq analyses of ...
Here we present a large-scale single-nucleus transcriptome dataset of the brain´s acute response to ischemic stroke. We dissect subtype-specific transcriptional signatures of stroke reactive neuroglia, compare subtype-specific astrocyte and oligodendrocyte lineage cell responses and contrast these changes...
Allow 30–60 days for the review process and 30 days for interaction with the Biostatistics Core to provide the dataset.” The processed single nucleus RNAseq data generated in this study can be freely viewed at http://web.hararilab.org/SNARE/. The 5xFAD mouse microglia data used in this...
#科研# 单细胞核RNA测序(single-nucleus RNA sequencing, snRNA-seq)[思考];10x Genomics和BD Rhapsody单细胞测序平台[思考] http://t.cn/A6UNpPAs
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
DroNC-seq 10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中...
虽然现存生物体中绝大多数作用于线粒体的蛋白质，是由细胞核DNA所制造，但这些基因中有一些可能是源于...
To characterize the transcriptional profile of myonuclei within myofibers, we developed single nucleus RNA-seq (snRNA-seq) experiments using a droplet-based platform on purified nuclei from different skeletal muscles of adult mice (Fig.1aand Supplementary Fig.1a–d). By using Seurat software25and ...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
DroNC-seq 10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中...