Single-nucleus RNA-sequencing (snRNA-seq) is emerging as a promising solution to scRNA-seq due to its reduced ionized transcription bias and compatibility with richer samples. This approach will provide an exciting opportunity for in-depth exploration of billions of formalin-fix...
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
Functional diversities within neurons and astrocytes in the adult rat auditory cortex revealed by single-nucleus RNA sequencing The mammalian cerebral cortex is composed of a rich diversity of cell types. Sensory cortical cells are organized into networks that rely on their function... A Gungor Aydin...
We provide an optimized single-nucleus RNA sequencing pipeline for clinical specimens with extensive quality assessments from tissue procurement through to single-cell transcriptomic analyses (Supplementary Fig.12). This tissue processing and interrogation work-flow is applicable to even limiting amounts of...
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel SingleNucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的 测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术 的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分...
Single cell RNA-sequencing (scRNASeq) has advanced our understanding of lung biology, but utility is limited by the need for fresh samples, loss of cell types by death or inadequate dissociation, and transcriptional stress responses induced during tissue digestion. Single nucleus RNASeq (snRNASeq)...
Finally, our findings indicate that snuclRNA-seq provides a valuable resource that can improve our understanding of the sequence of regulatory events that control cell fate leading to AD. Therefore, we are making available the nucleus-specific expression profile of the brains we analyzed through an...
Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain...
R. et al. Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons. Nat. Protoc. 11, 499–524 (2016). Article CAS PubMed PubMed Central Google Scholar Kalish, B. T. et al. Single-nucleus RNA sequencing of mouse auditory cortex reveals critical period triggers...