Single-Nucleus RNA Sequencing, Droplet by DropletResearchers describe a new single-cell expression profiling technique. Called DroNc-Seq, it merges an earlier platform with microfluidics, allowing massively parallel measurement of gene expression in structurally-complicated tissues.Broad Institute of MIT...
他们的研究成果发表在《iScience》杂志上,为我们理解脊椎动物视觉通路的进化提供了全新的视角。 在这项研究中,研究人员运用了单细胞核 RNA 测序(single-nucleus RNA sequencing,snRNA-seq)技术以及高分辨率空间转录组测序技术。他们选取了鸟类的视顶盖(optic tectum,OT)作为研究对象,这是鸟类视觉处理的关键亚皮质中枢之一...
Here we present a large-scale single-nucleus transcriptome dataset of the brain´s acute response to ischemic stroke. We dissect subtype-specific transcriptional signatures of stroke reactive neuroglia, compare subtype-specific astrocyte and oligodendrocyte lineage cell responses and contrast these changes...
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
Single-nucleus RNA-sequencing (snRNA-seq) has emerged as a powerful approach to interrogating the underlying transcriptional landscape of the cellularly-complex human brain. SnRNA-seq has been used to study AD in different cohorts and brain regions, including the entorhinal cortex15, temporal neocorte...
Single-nucleus RNA-sequencing (snRNA-seq) has emerged as a powerful approach to interrogating the underlying transcriptional landscape of the cellularly-complex human brain. SnRNA-seq has been used to study AD in different cohorts and brain regions, including the entorhinal cortex15, temporal neocorte...
Defining cellular and molecular identities within the kidney is necessary to understand its organization and function in health and disease. Here we demonstrate a reproducible method with minimal artifacts for single-nucleus Droplet-based RNA sequencing
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughpu...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha