RNA-sequencing from single nuclei. Proc Natl Acad Sci U S A. 2013;110:19802–7. CentralGrindberg RV et al (2013) RNA-sequencing from single nuclei. Proc Natl Acad Sci U S A 110:19802–19807 CentralKolodziejczyk AA, Kim JK, Svensson V, Marioni JC, Teichmann SA. The technology...
Finally, the method enables dissection of regulatory events at the single-cell level; pooling of 10 nuclei or 10 cells obscures some of the variability measured in transcript levels, implying that single nuclei and cells will be extremely useful in revealing the physiological state and ...
Research Highlight | 08 April 2022 Sequencing RNA isoforms in brain tissue Single-nuclei isoform RNA sequencing enables cell-type specific isoform analysis in frozen brain samples. Lei Tang Browse broader subjects Sequencing Browse RNA sequencing across other nature.com journals Previous...
lipid metabolism, and neuroimmune modulation. However, the cell-specific effect of variants in these genes is not fully understood. Here, we perform single-nucleus RNA-sequencing (snRNA-seq) on nearly 300,000 nuclei from the parietal cortex of AD autosomal dominant (APPandPSEN1...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
Herein, we performed single-nuclei RNA-sequencing (snRNAseq) to facilitate a better understanding of the complex pathobiological environment within the PAD limb and intercellular communication. METHODS: Gastrocnemius muscle specimens were collected from 20 patients with PAD and 12 non-PAD controls. ...
It serves as alternative to the use of protoplast isolation, which is currently a standard methodology for plant single-cell genomics, although it can be challenging for some plant tissues. We show the applicability of our nuclei isolation method by using different plant materials from several ...
single-nuclei suspensions. Additionally, different attempts resulted in inconsistent dissociation because the low amount of input tissue was barely visible and due to the manual component, it was difficult to balance complete dissociation with the destruction of cells. Based on these observations we ...
To understand how mutations in mybc3 causing sarcomere dysfunction result in the development of non-myocyte phenotypes such as fibrosis, mitral valve anomalies, and microvascular occlusion across species, we generated single nuclei RNA-sequencing datasets from feline, human and murine heart tissue ...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha