Single-Nucleus RNA Sequencing (SnRNA-seq) of Biopsy-Confirmed IgA Nephropathy Reveals Cell-Specific Transcriptomic Alterationdoi:10.1681/ASN.2024st8s6pa7Jeongmin ChoSeoul National University College of Medicine, Jongno-gu, Seoul, Korea (the Republic of);Sehoon Park...
#科研# 单细胞核RNA测序(single-nucleus RNA sequencing, snRNA-seq)[思考];10x Genomics和BD Rhapsody单细胞测序平台[思考] http://t.cn/A6UNpPAs
Single-nucleus RNA sequencing (snRNA-seq) measures gene expression in individual nuclei instead of cells, allowing for unbiased cell type characterization in solid tissues. We observe that snRNA-seq is commonly subject to contamination by high amounts of ambient RNA, which can lead to biased ...
Here, we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional diversity within multinucleated skeletal myofibers. Nuclei from mouse skeletal muscle were profiled across the lifespan, which revealed the emergence of distinct myonuclear populations in postnatal ...
The late embryonic development of the liver, a major metabolic organ, remains poorly characterized at single cell resolution. Here, we used single-nucleus RNA-sequencing (snRNA-seq) to characterize the chicken liver cells at 2 embryonic development time points (E14 and D1). We uncovered 8 cell...
in the context of PD have not yet been described. Recent technological advancements have facilitated more granulated analyses on the molecular phenotypes of PD at the single-cell level. Single-nucleus RNA sequencing (snRNA-seq) studies have examined cellular heterogeneity of the substantia nigra (SN...
Single-cell RNA sequencing (ScRNA-seq), single-nucleus RNA sequencing (SnRNA-seq), and Xenium-based spatial transcriptomics analyses were employed to evaluate the changes in mRNA expression in glial cells and neurons. Results From the ScRNA-seq and SnRNA-seq data, 31,390 glial cells and 48,...
Here, we used single-nucleus RNA sequencing (snRNA-seq) to reveal distinct populations of cardiomyocytes in regenerating neonatal and non-regenerative later-stage hearts. We show that a distinct immature population of cardiomyocytes enters the cell cycle in response to injury. Transcriptome analysis of...
In comparison with single-cell RNA sequencing (scRNA-seq) library generated with live cells from freshly isolated human islets, the snRNA-seq library displayed comparable endocrine cellular composition and cell type signature gene expression. However, between these two types of libraries, differential ...
Recent advances in single-nucleus RNA sequencing (snRNA-seq) have provided an unprecedented ability to disentangle cellular-level transcriptomic alterations and heterogeneity in Alzheimer’s disease (AD)1,2. Early differential expression with neuropathological AD progression have been found to be cell-...