Here, we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional diversity within multinucleated skeletal myofibers. Nuclei from mouse skeletal muscle were profiled across the lifespan, which revealed the emergence of distinct myonuclear populations in postnatal ...
Single-nucleus RNA-sequencing (snRNA-seq) has emerged as a powerful approach to interrogating the underlying transcriptional landscape of the cellularly-complex human brain. SnRNA-seq has been used to study AD in different cohorts and brain regions, including the entorhinal cortex15, temporal neocorte...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
Cells, as the fundamental units of life, contain multidimensional spatiotemporal information. Single-cell RNA sequencing (scRNA-seq) is revolutionizing biomedical science by analyzing cellular state and intercellular heterogeneity. Undoubtedly, single-cell transcriptomics has emerged as one of the most vibra...
#科研# 单细胞核RNA测序(single-nucleus RNA sequencing, snRNA-seq)[思考];10x Genomics和BD Rhapsody单细胞测序平台[思考] http://t.cn/A6UNpPAs
William et al. constructed a high-resolution molecular landscape of the cellular subtypes and spatial communities that compose PDAC using single-nucleus RNA sequencing (snRNA-seq) and whole-transcriptome DSP. This study was performed to reveal possible interactions among malignant cells, CAFs, and immu...
Recent advances in single-nucleus RNA sequencing (snRNA-seq) have provided an unprecedented ability to disentangle cellular-level transcriptomic alterations and heterogeneity in Alzheimer’s disease (AD)1,2. Early differential expression with neuropathological AD progression have been found to be cell-...
Single-nucleus RNA sequencing (snRNA-seq) is used as an alternative to single-cell RNA-seq, as it allows transcriptomic profiling of frozen tissue. However, it is unclear whether snRNA-seq is able to detect cellular state in human tissue. Indeed, snRNA-seq analyses of human brain samples hav...
An in-depth exploration of cardiac disease using single-cell technologies contributing to predicting disease, therapeutic target discovery, and stratifying patients [224,225,226]. The work from Kanemaru et al. employed sc-RNA-seq, single-nucleus RNA sequencing (snRNA-seq), snATAC-seq, and spatial...
Both the single-nucleus (snRNA-seq) and single-cell (scRNA-seq) RNA-sequencing datasets were produced using cultured human islet samples obtained from the same donor. In particular, we generated single-cell and single-nucleus libraries using 4 technical replicates for each method. Gene counts were...