为了验证鉴定小鼠interneurons的Dlx1/2调控网络,作者分析了人脑的sNuc-Seq(Single nuclei RNA-Seq)数据集。 在人类脑sNuc-Seq数据集上,SCENIC也鉴定出由DLX1/2强烈驱动的interneurons细胞群,该群具有与小鼠相同的motif,并且识别出一组保守的靶标,包括DLX1本身。 接下来,作者将这种跨物种分析扩展到其他细胞类型。与基...
Single-nuclei isolationsnRNA-seqArabidopsis thaliana seedlingsflower developmentantherSingle-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nuclei isolation combined with nanowell-based library ...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with di...
在一篇发表于《RAPID COMMUNICATION》的“单细胞核RNA测序比单细胞RNA测序可以发现更多小鼠肾脏纤维细胞稀少的细胞类型和未知的细胞状态”文章中提到,采用 DropSeq,DroNc-seq和10xChromium平台(平台差异详见Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems)分别进行单细胞和单细胞核...
Single-nuclei RNA-seq cleaning The gene expression matrices from all samples were combined in R independently for further processing using the Seurat (version 2.20, 2.30) pipeline. We processed the gene expression quantified for the pre-mRNA and mRNA references in parallel. We removed most of the...
First, we used single-nucleus RNA sequencing to estimate RNA velocity. Compared to single-cell sequencing, snRNA-seq is more amenable to transcriptomic profiling of postmortem samples because isolated nuclei are intact in frozen tissues29. Moreover, dissociating whole cells from the brain is ...
Our nuclei isolation procedure can be applied in different plant species and tissues, thus expanding the toolkit for plant single-cell genomics experiments. SIGNIFICANCE STATEMENT We introduce an optimized plant nuclei isolation procedure followed by single nuclei RNA-seq that can be applied to ...
UsingsinglenucleiforRNA-seqtocapturethetranscriptomeof postmortemneurons SugunaRaniKrishnaswami 1,9 ,RashelVGrindberg 2,9 ,MarkNovotny 1 ,PratapVenepally 3 , BenjaminLacar 4 ,KunalBhutani 1 ,SaraBLinker 4 ,SonPham 4 ,JenniferAErwin 4 ,JeremyA Miller 5 ,RebeccaHodge 5 ,JamesKMcCarthy 1 ,Marti...
To systematically assess the differences between high-throughput single-cell and single-nuclei RNA-seq approaches, we compared Drop-seq and DroNc-seq, two ... A Selewa,R Dohn,H Eckart,... - 《Scientific Reports》 被引量: 0发表: 2020年 METHODS FOR DETERMINING SPATIAL AND TEMPORAL GENE EXPRE...