GEM-X technology substantially improves gene and transcript sensitivity. Nuclei were isolated from a section of an adult CD-1 mouse brain using the Chromium Nuclei Isolation Kit and enriched to remove debris. Nuclei were loaded separately onto a Next GEM Chip G (5,000 nuclei) and a GEM-X 3...
GEM-X technology substantially improves gene and transcript sensitivity. Nuclei were isolated from a section of an adult CD-1 mouse brain using the Chromium Nuclei Isolation Kit and enriched to remove debris. Nuclei were loaded separately onto a Next GEM Chip G (5,000 nuclei) and a GEM-X 3...
Raw sequence reads from single nuclei RNA-seq (snRNA-seq) were processed and after QC filtering, cells were clustered using Seurat. Clusters were annotated using singleR. Results: In PcAT and EAT, >7000 cells were successfully extracted with 73755 and 48750 mean snRNA-seq reads per cell, ...
The single-cell RNA sequencing (scRNA-seq) is an emerging technology that provides a powerful tool for identifying cell types and cell states by analyzing the expression profile of an individual cell's transcriptome [30,31]. This technique has been successfully utilized to gain insights into the ...
In this study, single-cell RNA sequencing (scRNA-seq) was employed to analyze the rhd6 and gl2 mutant root epidermis at high resolution. The analysis revealed distinct molecular phenotypes: rhd6 mutants lacked root-hair cells, with some cells adopting nonhair characteristics, while gl2 mutants ...
S. et al. MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices. Nat. Methods 16, 619–626 (2019). Article CAS PubMed PubMed Central Google Scholar Gaublomme, J. T. et al. Nuclei multiplexing with barcoded antibodies for single-nucleus genomics. Nat. ...
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1k Nuclei from BJ Fibroblast Euploid Cell Line 0.99 0.04 1k Cells from BJ Fibroblast Euploid Cell Line 0.97 0.04 BJ with 10% MKN-45 Spike-In 0.98 0.05 1k BJ with 1% MKN-45 Spike-In 0.97 0.04A cell can have high DIMAPD due to underlying biological reasons or due to the library prepara...
assignment. Our approach, entitledScNaUmi-seq(Single-cell Nanopore sequencing with UMIs), enables the analysis of splicing and sequence variation at the single-cell level with the 10x Genomics Chromium system. This is illustrated with data on alternative splicing and RNA editing in embryonic mouse ...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha