Ziegenhain C, Vieth B, Parekh S, et al. Comparative analysis of single-cell RNA sequencing methods[J]. Molecular cell, 2017, 65(4): 631-643. e4.
单细胞测序技术(single cell sequencing)是指在单个细胞水平上,对基因组、转录组、表观组进行高通量测序分析的一项新技术,它能够弥补传统高通量测序的局限性,揭示单个细胞的基因结构和基因表达状态,反映细胞间的异质性。2013年,单细胞测序技术被《Science》将其列为年度最值得关注的六大领域榜首;2015年再次登上Science...
题目:Single-cell RNA sequencing ofperipheral blood mononuclear cells from acute Kawasaki disease patients 时间:2021年9月 期刊:Nature communications (IF: 12.298) 链接:https://doi.org/10.1016/j.immuni.2020.07.009 作者利用单细胞RNA以及BCR/TCR测序对急性KD患者PBMCs进行分析,通过静脉注射免疫球蛋白(IVIG)...
10X Genomics运用微流控系统进行细胞分选，带有Barcode的Gel Beads 与水象的细胞和酶相结合，进入油相后...
高校开学已有些时间,此次BioSciTool更新带来基于10X Genomics测序CellRange输出结果的单细胞常规转录组(SingleCell RNA-Seq)数据分析程序。多个程序主要参考10X Genomics数据格式和Seurat数据分析流程,允许用户执行数据QC、细胞过滤、线粒体基因占比统计、Feature过滤、高变Feature鉴定、PCA分析及JackStraw对PCA的选择、细胞UMA...
2020 年4 月,西班牙科学家在 Nature 杂志上发表了一篇论文Benchmarking single-cell RNA-sequencing protocols for cell atlas projects论述了不同的单细胞测序技术的差异,文章比较了13种常用scRNA-seq和单核RNA-seq实验方案。比较分析显示实验方案的性能存在明显差异。这些实验方案在文库的复杂性及其检测细胞类型标记的...
来自单个细胞的所有cDNA,源自单个Gel Bead,将具有相同的10X条形码,能够将每个转录本映射回其起源细胞。然而,单个凝胶珠上的单个寡核苷酸具有不同的UMIs,这使得每个cDNA分子具有独特的随机12碱基序列。这允许在下游处理过程中消除任何PCR重复。 然后将条形码cDNA用于制备测序文库,其中包括sequencing adapters的添加和PCR扩增...
Single-Cell RNA Sequencing Resolves Molecular Relationships Among Individual Plant Cells Journal: Plant physiology Impact factor: 7.479 Published: 04 February 2019 Background Different cell types in organisms result from varying gene expression, critical for multicellular functions.Single-cell RNA sequencing ...
Single-cell RNA sequencing can reveal the variability between cells, which can help the population quickly adapt to the changing environment. CD Genomics provides comprehensive single-cell RNA sequencing services.
Precursor states of multiple myeloma (MM) and its native tumor microenvironment need in-depth molecular characterization to better stratify and treat patients at risk. Using single-cell RNA sequencing of bone marrow cells from precursor stages, monoclona