Herein, we performed single-nuclei RNA-sequencing (snRNAseq) to facilitate a better understanding of the complex pathobiological environment within the PAD limb and intercellular communication. METHODS: Gastrocnemius muscle specimens were collected from 20 patients with PAD and 12 non-PAD controls. ...
Here, we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional diversity within multinucleated skeletal myofibers. Nuclei from mouse skeletal muscle were profiled across the lifespan, which revealed the emergence of distinct myonuclear populations in postnatal ...
In this study, we used single-cell RNA-sequencing (scRNAseq) to define the molecular logic by which SGN subtypes diversify in the mouse. Our detailed analysis of transcriptional dynamics of SGNs from several embryonic and perinatal stages indicates that neuronal subtypes successively emerge during HC...
By analyzing the results of scRNA-Seq on cells isolated from JEV-infected regions in mouse brains, we found the proportion of neurons did not show significant changes in the brains of mice with mild symptoms; however, there was a substantial decrease in the proportion of neurons in the brains...
Single-cell, thiol-(SH)-linked alkylation of RNA for metabolic labelling sequencing (scSLAM-seq) pioneered the application of the metabolic labeling method at the single-cell level [32]. This breakthrough identified DEGs in mouse fibroblasts infected and uninfected with cytomegalovirus (CMV) by di...
Single-cell RNA-Seq reveals transcriptomic heterogeneity and post-traumatic osteoarthritis-associated early molecular changes in mouse articular chondrocytes. Cells. 2021;10(6):1462. Article CAS PubMed PubMed Central Google Scholar Karsenty G, Kronenberg HM, Settembre C. Genetic control of bone ...
Skeletal muscle development is a multistep process whose understanding is central in a broad range of fields and applications, from the potential medical value to human society, to its economic value associated with improvement of agricultural animals. S
These cells were used for single-cell RNA sequencing (scRNA-seq). The functional role of CFs was analyzed with a mouse knock-out model and their regulatory mechanisms with assay for transposase-accessible chromatin sequencing (ATAC-seq) and in vitro assays to validate the hypothesis generated. D...
Figure S1. Information on Monkeys and Quality Control of Single-Cell RNA-Seq, Related toFigure 1 (A) Information summary of the monkeys analyzed in this study. (B) H&E-stained sections of young and old monkey ovaries. Yellow arrowheads indicate the atretic follicles. Scale bar, 100 μm....
[38] were found in both single-cell and bulk RNA-seq data. Enrichment analysis of significantly changed MRTF transcriptional target genes suggested regulations of smooth muscle proliferation, actin cytoskeleton, integrin cell surface interactions, and extracellular matrix organization (Additional file1: ...