Single-cell RNA-seq is an unbiased approach that has extended our understanding of heterogeneous tissues, including mouse and human embryonic gonads.18,19,20We reasoned that analysis of stage-specific gene expression profiles of individual spermatogenic cells could provide unbiased and novel insights int...
Recently, the transcript diversity from gastrulation through organogenesis of mammalian embryos has been studied by bulk-cell RNA-seq [8,9,10,11]. However, few studies have focused on cellular heterogeneity among organs at single-cell resolution, especially during the early stages of organogenesis. ...
In this study, we used single-cell RNA-sequencing (scRNAseq) to define the molecular logic by which SGN subtypes diversify in the mouse. Our detailed analysis of transcriptional dynamics of SGNs from several embryonic and perinatal stages indicates that neuronal subtypes successively emerge during HC...
Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both poly
Seven days after ligation, single-cell RNA sequencing of nine left carotid arteries (LCA) from the PCL group (10,262 cells) or control group (14,580 cells) was applied and a single-cell atlas of gene expression was constructed. The integrated analysis identified 15 distinct carotid cell ...
Single cell RNA seq GLP-1 PPG-cells L-cells Mass spectrometry 5-HT 1. Introduction Enteroendocrine preproglucagon-expressing PPG-cells (traditionally known as L-cells) secrete the gut hormones glucagon-like peptide 1 (GLP-1) and peptideYY (PYY) and are important regulators of glucose metabolism...
And then the cellular atlas of RILI liver was generated by profiling 9,641 cells isolated from X-ray irradiated mice livers and control ones from RILI mice model using single-cell RNA sequencing (scRNA-seq). Seven cell types were identified, including B cells, natural killer cells, T ...
Single-cell RNA-seq revealed diverse cell types in the mouse placenta at mid-gestationXiaobo Zhou a bYingchun Xu bShengnan Ren bDong Liu bNingjie Yang bQian Han bShuangbo Kong b cHaibin Wang b cWenbo Deng b cHongbo Qi a
Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity The hypothalamus is one of the most complex brain structures involved in homeostatic regulation. Defining cell composition and identifying cell-type-specif... R Chen,X Wu,J Lan,... - 《Cell Reports》 被引量: 31发表: 2017年 Neuroglial ...
5.Single-cell transcriptional landscape of developing vMB 接着他们分选出了绿色的单个细胞,gathered cells from Cre-CREST mouse brains at designated time points and conducted scRNA-seq。他们termed this workflow snapshotting CREST (snapCREST)。首先他们先进行了单细胞转录组的分析,鉴定了主要的细胞类型,以及...