aExperiment schematic. Single-cell RNA sequencing was performed on total and depleted (CD45−/Ter119−) bone marrow cells.bProjection of data onto two dimensions usingt-distributed stochastic neighbor embedding (tSNE63) indicates that bone marrow population structure is preserved in biological repli...
Single-cell RNA-seq is an unbiased approach that has extended our understanding of heterogeneous tissues, including mouse and human embryonic gonads.18,19,20We reasoned that analysis of stage-specific gene expression profiles of individual spermatogenic cells could provide unbiased and novel insights int...
Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both poly
Single cell RNA seq GLP-1 PPG-cells L-cells Mass spectrometry 5-HT 1. Introduction Enteroendocrine preproglucagon-expressing PPG-cells (traditionally known as L-cells) secrete the gut hormones glucagon-like peptide 1 (GLP-1) and peptideYY (PYY) and are important regulators of glucose metabolism...
Single-cell RNA-seq revealed diverse cell types in the mouse placenta at mid-gestationXiaobo Zhou a bYingchun Xu bShengnan Ren bDong Liu bNingjie Yang bQian Han bShuangbo Kong b cHaibin Wang b cWenbo Deng b cHongbo Qi a
Single cell RNA-sequencing (scRNASeq) has advanced our understanding of lung biology, but utility is limited by the need for fresh samples, loss of cell types by death or inadequate dissociation, and transcriptional stress responses induced during tissue digestion. Single nucleus RNASeq (snRNASeq)...
SMART-Seq Mouse BCR (with UMIs) enables users to analyze B-cell receptor (BCR) repertoires from 10 ng–1 µg high-integrity (RIN>7) bulk total RNA samples from spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs) or RNA extracted from mouse whole blood (RNA input ≥100...
细胞的培养采用公司专利产品小鼠关节软骨细胞培养试剂盒(MouseBonePrimaCell:NormalArticularChondrocytesCatNo.3-4022)来优化目的细胞生长条件,以降低杂细胞(如脂肪细胞、纤维细胞等)的污染,同时保证质量的稳定。在公司技术部标准的操作流程下,小鼠关节软骨细胞可以保持原代细胞的分化状态,进而可以用于评估体外药物模型系统和...
细胞培养方法: 小鼠关节软骨细胞培养试剂盒 Mouse Bone PrimacellTM : Normal Articular Chondrocytes 1 、细胞传代:细胞密度达到 80-90% 时即可传代 ① 弃去培养上清,用 PBS 或生理盐水清洗 1-2 次; ② 加入 2ml0.
Mouse GGT小鼠(Mouse)γ谷氨酰转移酶(GGT)-NEWA,96T/48T,大包装可定制, 产品货源充足,质量保证,下单即可发货,部分产品需提前订购,下单后1-2个工作日内安排出库。 详细内容 公司简介 ---仅供科研试验使用,不得用于临床或食用--- Mouse GGT小鼠(Mouse