Mesenchymal stem cells (MSCs) are a common kind of multipotent cell in vivo, but their heterogeneity limits their further applications. To identify MSC subpopulations and clarify their relationships, we performed cell mapping of bone-marrow-derived MSCs through single-cell RNA (scRNA) sequencing. In...
Here we show that transfer learning鈥攖he branch of machine learning that concerns passing information from one domain to another鈥攃an be used to efficiently map bone marrow biology between species, using data obtained from single-cell RNA sequencing. We first trained a multiclass logistic ...
However, single-cell resolution datasets of neutrophil transcriptional landscapes that include both sexes are still lacking. Here, we generated a single-cell RNA-seq dataset of primary neutrophils from 3-month-old female and male adult mouse bone marrow with biological replicates (n = 2 per ...
ScRNA-seq has four major advantages over bulk RNA-seq in biological research. First, this approach can reveal cellular heterogeneity or specific subclusters; second, it can depict the trajectories of different cell states, transitions, and differentiation in tissue during physiological and pathological ...
Fig. 3: Single-cell transcriptome profiling of the bone marrow niche in response to chemotherapy Notch ligands from vascular endothelial cells 我们的分析显示,Notch配体Dll1和Dll4在VE-Cad+细胞中特异性表达(图2f)。反转录定量PCR分析证实,与血管周细胞和骨系细胞相比,Dll1和Dll4在血管内皮细胞亚群中的高...
hematopoietic stem cells, bone marrow stromal cells and others. Our future work will be focused on single-cell based data analyses. 5. Conclusion E'jiao effectively improved the recovery of blood deficiency induced by 5-FU via the promotion in the proliferation of bone marrow nucleated cells (...
49 Single-cell and bulk-RNA sequencing studies have additionally highlighted increases in MDL-1 (CLEC5A) expression in inflamed intestinal tissue compared with control-matched non-inflamed tissue.50,51 Although human and murine correlates of bone marrow OCPs are poorly defined, bulk-RNA sequencing ...
目标:利用文章中描述的工具和方法,复现文中主要的scRNA-seq分析图表 前言 最有效的学习方法就是在实践中学习,今天开始要尝试复现的文章是A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia,该文章于2019年发表在Cell杂志上。文章的分析代码并未开源,因此只能根据原文描述来尽量复现其结果。
We performed single-cell RNA sequencing to profile 39,288 cells from 6 bone marrow (BM) aspirates including 5 NK-AML (M4/M5) patients and 1 healthy donor. The single-cell transcriptome atlas and gene expression characteristics of each cell population in NK-AML (M4/M5) and healthy BM were...
Specifically, bone marrow mesenchymal stroma cells (BM-MSCs), along with periosteal cells, have been postulated as the cells driving the bone formation during regeneration [6]. At the translational level, human BM-MSCs (hBM-MSCs) have been thoroughly used as a valid cell model in bone regenera...