In this paper, we describe a single-cell RNA sequencing (scRNA-seq) dataset that defines vascular and vessel-associated cell types and subtypes in mouse brain and lung. The dataset contains 3,436 single cell transcriptomes from mouse brain, which formed 15 distinct clusters corresponding to cell...
The mammalian brain contains many specialized cells that develop from a thin sheet of neuroepithelial progenitor cells. Single-cell transcriptomics revealed hundreds of molecularly diverse cell types in the nervous system, but the lineage relationships b
RNA isoforms influence cell identity and function. However, a comprehensive brain isoform map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes, developmental time points and species. For 72% of genes, full-length isofo
Single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to characterize individual cells in depth (Kolodziejczyk et al., 2015); it was recently utilized in the intestine to identify cell types (Grün et al., 2015) and sub-populations of intestinal stem cells (Yan et al., ...
While the hypothalamus is a well-conserved brain region that controls these homeostatic and survival-related behaviors, little is known about the intrinsic features of hypothalamic aging. Here, we perform single-nuclei RNA sequencing of 40,064 hypothalamic nuclei from young and aged female mice. We...
继续打卡,最近上线的一篇在Nature Method上的由国人开发的一个单细胞谱系示踪的工作。题目是:Comprehensivespatiotemporalmapping of single-cell lineages in developing mouse brain by CRISPR-based barcoding。也是今天BioArt推送的公众号文章,因为加了spatiotemporal的关键词,感觉文章还不错呢。
(RNA-seq). The first macro-area is the cerebral cortex (Cx), while the second one includes the midbrain, the diencephalon and the hippocampus (M). The Ids-ko mouse used in these experiments has been extensively studied and characterized ...
Cluster 13 cells expressing the interferon-related module were roughly twice as abundant in 5XFAD brains (0.9%) than in controls (0.4%), and we also observed a similar ∼doubling of this population using single-cell RNA-seq in the PS2APP model (unpublished data). Our gene sets therefore ...
Neonatal astrocytes snRNA-seq: Single nuclei sequencing WT: Wild type References Keren-Shaul H, Spinrad A, Weiner A, Matcovitch-Natan O, Dvir-Szternfeld R, Ulland TK, et al. A unique microglia type associated with restricting development of Alzheimer’s disease. Cell. 2017;169(7):127...
Different types of spiral ganglion neurons (SGNs) are essential for auditory perception by transmitting complex auditory information from hair cells (HCs) to the brain. Here, we use deep, single cell transcriptomics to study the molecular mechanisms that govern their identity and organization in mice...