The recent advent of massively parallel high-throughput droplet-based profiling techniques have further hastened the adaptation of single-cell RNA-seq in cataloging cells in the central and peripheral nervous system of adults11,12,13,14,15. Here, we use Drop-seq to characterize the cellular ...
Different types of spiral ganglion neurons (SGNs) are essential for auditory perception by transmitting complex auditory information from hair cells (HCs) to the brain. Here, we use deep, single cell transcriptomics to study the molecular mechanisms that govern their identity and organization in mice...
In addition to the newmouse braincell data, today's release includes data at the single cell level from human and macaque brains, through collaborative projects with teams at UW Medicine and the Swedish Neuroscience Institute. These precious data sets provide researchers with insight into differences...
BrainPalmSeq: A curated RNA-seq database of palmitoylating and de-palmitoylating enzyme expression in the mouse braindoi:10.1101/2021.11.23.468539Angela R WildPeter HoggStephane FlibotteShernaz BamjiCold Spring Harbor Laboratory
A large-scale application of the “stacked modeling” approach for chromatin state discovery previously provides a single “universal” chromatin state annotation of the human genome based jointly on data from many cell and tissue types. Here, we produce an analogous chromatin state annotation for m...
Table S1. Preparation of Region-Specific Single-Cell Suspensions from Acute Brain Tissue, Related to Figure 1 Data S1. Region-Specific Tissue Volumes Processed as Input for Drop-Seq, Related to Figure 1 Data S2. ICA-Based Global Clustering Is Robust, Related to Figure 1 Data S3. Presentation...
Cluster 13 cells expressing the interferon-related module were roughly twice as abundant in 5XFAD brains (0.9%) than in controls (0.4%), and we also observed a similar ∼doubling of this population using single-cell RNA-seq in the PS2APP model (unpublished data). Our gene sets therefore ...
We further performed cell-type and brain-region enrichment analysis using published single-cell RNA-seq (scRNA-seq) data and Allen in situ hybridization (ISH) data from the developing mouse brain. Our study provided an unbiased dataset for transcriptional alterations in the mouse brain after PS, ...
A few non-neuronal cell types were also identified in the MGE. In addition, single-cell RNA-seq data of in vitro ES-differentiated cells were analyzed and compared to in vivo embryonic MGEs, revealing similar gene expression profiles, however, with some differences (Fig. 1B). Results ...
A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues into single-cell suspensions. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that dissociation can ha