Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain. Genes Dev. 35, 1190–1207 (2021). Article PubMed PubMed Central Google Scholar Li, Q. et al. Developmental heterogeneity of microglia and brain myeloid cells revealed by deep single-cell RNA sequencing. ...
In addition to the newmouse braincell data, today's release includes data at the single cell level from human and macaque brains, through collaborative projects with teams at UW Medicine and the Swedish Neuroscience Institute. These precious data sets provide researchers with insight into differences...
Different types of spiral ganglion neurons (SGNs) are essential for auditory perception by transmitting complex auditory information from hair cells (HCs) to the brain. Here, we use deep, single cell transcriptomics to study the molecular mechanisms that govern their identity and organization in mice...
基本共性: 1、小鼠神经星形胶质细胞提取物 Mouse Brain: Normal Nerve AstrocytesDerivatives所有的细胞表面均有由磷脂双分子层与镶嵌蛋白质及糖被构成的生物膜,即细胞膜。 2、所有的细胞都含有两种核酸:即DNA与RNA。 3、作为遗传信息复制与转录的载体。 4、作为蛋白质合成的机器—核糖体,毫无例外地存在于一切细胞内...
BrainPalmSeq: A curated RNA-seq database of palmitoylating and de-palmitoylating enzyme expression in the mouse braindoi:10.1101/2021.11.23.468539Angela R WildPeter HoggStephane FlibotteShernaz BamjiCold Spring Harbor Laboratory
该分析证明了使用RAPIDS加速使用单个 GPU 分析 100 万个细胞的单细胞 RNA 序列数据。然而,实验只处理了前 100 万个细胞,而不是整个 130 万个细胞。因此,在单细胞 RNA 数据的工作流中处理所有 130 万个细胞的时间几乎是单个 V100 GPU 的两倍。另一方面,相同的工作流在单个 NVIDIA A100 40...
这为基于PCR的DNA和RNA定量方法带来了革命性的变革。在实时荧光定量PCR (qPCR)中,反应以循环中*检测到目标扩增的时间点为特征,而非在一定循环数后目标分子累计的扩增量。目标核酸的起始拷贝数越高,则会越快观察到荧光的显著增加。相反,终点法检测(也称 “读板检测”)测量的是PCR循环结束时累计的PCR产物量。
A large-scale application of the “stacked modeling” approach for chromatin state discovery previously provides a single “universal” chromatin state annotation of the human genome based jointly on data from many cell and tissue types. Here, we produce an analogous chromatin state annotation for m...
Table S1. Preparation of Region-Specific Single-Cell Suspensions from Acute Brain Tissue, Related to Figure 1 Data S1. Region-Specific Tissue Volumes Processed as Input for Drop-Seq, Related to Figure 1 Data S2. ICA-Based Global Clustering Is Robust, Related to Figure 1 Data S3. Presentation...
We found two major neural cell populations that were further characterized into different progenitor populations and neuronal classes. A few non-neuronal cell types were also identified in the MGE. In addition, single-cell RNA-seq data of in vitro ES-differentiated cells were analyzed and compared...