Single-cell RNA-seqSingle-nucleus RNA-seqPost-mortemSingle-cell genomicsPurpose of Review This review summarizes some of the most recent studies on single-cell resolution sequencing of the post-mortem human brain and the application of these techniques for the study of psychiatric and neurological ...
The human cortex comprises diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of human brain development, we performed single-c
Although placental cells have been traditionally classified as described above, the extent to which it is useful to define subtypes of trophoblast cells and stromal cells and the relationships between cell subtypes and functions remain unclear. Single-cell RNA-seq has been a powerful tool for the i...
Each panel shows for each bin the average relative expression in each of three normal brain cell types (y axis) based on data from the Barres laboratory RNA-seq database9,18: mice oligodendrocyte progenitor cells (mOPC, top), mouse neurons (mNeurons, middle), and human neurons (hNeurons,...
Single-cell analysis of the adult human brain is facilitated by improved methods for RNA-seq and hypersensitive-site mapping. Detailed characterization of the cell types in the human brain requires scalable experimental approaches to examine multiple asp
J. DoubletFinder: doublet detection in single-cell RNA sequencing data using artificial nearest neighbors. Cell Syst. 8, 329–337 (2019). Article CAS PubMed PubMed Central Google Scholar Wang, D. et al. Comprehensive functional genomic resource and integrative model for the human brain. ...
RNA isoforms influence cell identity and function. However, a comprehensive brain isoform map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes, developmental time points and species. For 72% of genes, full-length isofo
these analyses used cortical-level bulk data, and the need for greater resolution led us to characterize heterogeneity of human microglia at the single-cell level. Recently, studies of single nucleus RNA sequencing from human brain reported either no detectable population structure in microglial cells...
Glioblastoma (GBM) is the most common primary brain cancer in adults and is notoriously difficult to treat because of its diffuse nature. We performed single-cell RNA sequencing (RNA-seq) on 3,589 cells in a cohort of four patients. We obtained cells from the tumor core as well as surroun...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which