Single-cell RNA-seqSingle-nucleus RNA-seqPost-mortemSingle-cell genomicsPurpose of Review This review summarizes some of the most recent studies on single-cell resolution sequencing of the post-mortem human brain and the application of these techniques for the study of psychiatric and neurological ...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which
Each panel shows for each bin the average relative expression in each of three normal brain cell types (y axis) based on data from the Barres laboratory RNA-seq database9,18: mice oligodendrocyte progenitor cells (mOPC, top), mouse neurons (mNeurons, middle), and human neurons (hNeurons,...
these analyses used cortical-level bulk data, and the need for greater resolution led us to characterize heterogeneity of human microglia at the single-cell level. Recently, studies of single nucleus RNA sequencing from human brain reported either no detectable population structure in microglial cells...
J. DoubletFinder: doublet detection in single-cell RNA sequencing data using artificial nearest neighbors. Cell Syst. 8, 329–337 (2019). Article CAS PubMed PubMed Central Google Scholar Wang, D. et al. Comprehensive functional genomic resource and integrative model for the human brain. ...
We performed single-cell RNA-seq of 2,579 individual cells. After filtration, we retained 2,167 individual cells, including 1,490 FGCs and 677 somatic cells, for the subsequent analysis (Figure 1A). On average, we obtained approximately 1.9 million reads for each individual cell. The average...
Cell type diversity Functional clustering Editorial Summary RNA-seq survey of early brain development Advances in single-cell RNA-sequencing technology are making it possible to identify the many different types of cell that participate in the assembly of brain structures. Xiaoqun Wang and colleagues co...
先与RNA-seq的数据相结合,首先计算每个细胞亚类中单个核的可及性,将255828个远端cCRE连接到14861个假定的靶基因上,结果是在500kb内总共有1661975个gene-cCRE对。接下来,确定cCRE的亚群,其可及性与假定靶基因的表达呈正相关,因此可以在神经元或非神经元类型中作为推测的增强子(图2F,底部)。总共揭示了265049对...
The human cortex comprises diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of human brain development, we performed single-cell RNA-sequencing across regions of the deve...
We applied our liver single-cell dissociation protocol and scRNA-seq technology to identify resident liver cells in TLHs from NDD liver transplant donors (donor characteristics found in Supplementary Table1). Importantly, NDD grafts are subjected to the systemic inflammation that accompanies brain death...