hippocampus is important for further study of memory functions and neurological diseases. Single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequencing (snRNA-seq) are powerful tools for deciphering the cellular heterogeneity and function of the hippocampus as well as its dysfunction in ...
Single-cell RNAseqHippocampusLearning and memoryCognitive declineDementiaExercise has been recognized as a beneficial factor for cognitive health, particularly in relation to the hippocampus, a vital brain region responsible for learning and memory. Previous research has demonstrated that exercise-mediated ...
Single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequencing (snRNA-seq) are powerful tools for deciphering the cellular heterogeneity and function of the hippocampus as well as its dysfunction in diseases. A single cell is the smallest functional unit within an organism and maintains ...
1 Single-cell RNA-seq information and molecular diversity of single cells. a, Scheme of bioinformatic analysis. b, Expression of known markers shown using the same layout as in Fig. 1b. Grey, no expression; red, relative expression. c, Heat map showing the expression level and identity of...
Single-cell RNA-seq with waterfall reveals molecular cascades underlying adult neurogenesis. Cell Stem Cell 17, 360–372 (2015). Article PubMed PubMed Central CAS Google Scholar Suh, H. K. et al. In vivo fate analysis reveals the multipotent and self-renewal capacities of Sox2+ neural stem...
These samples were subjected to single-cell RNA sequencing (ScRNA-seq), single-nucleus RNA sequencing (SnRNA-seq), and Xenium-based (mouse brain panel, 247 genes) spatial transcriptomics analyses. We analysed 31,390 glial cells and 48,221 neuronal nuclei from the ScRNA-seq and SnRNA-seq data...
To induce TLE, 200 nl of kainic acid (KA) was stereotactically injected into the hippocampal CA1 region of mice, followed by a 7-day postinjection period. Single-cell RNA sequencing (ScRNA-seq), single-nucleus RNA sequencing (SnRNA-seq), and Xenium-based spatial transcriptomics analyses were...
short-termmemorytolong-termmemory1,2.Hereweusesingle-cellRNAsequencing andassayfortransposase-accessiblechromatinusingsequencing(ATAC–seq) analysistoillustratethecelltypes,celllinage,molecularfeaturesandtranscriptional regulationofthedevelopinghumanhippocampus.Usingthetranscriptomesof 30,416cellsfromthehumanhippocampusat...
Single-cell RNA-seq with waterfall reveals molecular cascades underlying adult neurogenesis. Cell Stem Cell 17, 360–372 (2015). CAS PubMed Google Scholar Artegiani, B. et al. A single-cell RNA sequencing study reveals cellular and molecular dynamics of the hippocampal neurogenic niche. Cell ...
RNAscope approach (Fig.5c–f). To understand the distinct cell types responsible for upregulation ofSgk1following learning, we performed a single-nuclei RNA seq using a droplet capture independent split-pool barcoding approach (Fig.5g). We identified 15 clusters of cells from the dorsal ...