图一:Drop-Seq 哈佛医学院Steven McCarroll领导的团队将微流控技术引入单细胞RNA-seq方法中,开发了Drop-seq技术,且该项技术于2015年发表于《Cell》杂志上(Macosko et al., 2015)。Drop-seq技术利用微流体装置将带有细胞条形码的微珠和细胞一起装入微滴。这些液滴在一个小型设备上生成,沿着一根头发宽的槽道流动...
Here we use deep single-cell RNA sequencing (scRNAseq) together with other experimental approaches to investigate fate choices, lineage relationships and molecular determinants during early stages of sensory neurogenesis in the mouse. Overall, our data provide insights into the structure of the Waddingto...
Single-cell RNA sequencing (scRNA-seq) has enabled researchers to study gene expression at a cellular resolution. However, noise due to amplification and dropout may obstruct analyses, so scalable denoising methods for increasingly large but sparse scRNA-seq data are needed. We propose a deep count...
The single-cell RNA sequencing (scRNA-seq) is an emerging technology that provides a powerful tool for identifying cell types and cell states by analyzing the expression profile of an individual cell's transcriptome [30,31]. This technique has been successfully utilized to gain insights into the ...
题目:Diversification of molecularly defined myenteric neuron classes revealed by single-cell RNA sequencing 时间:2020年12月 期刊:Nature Neuroscience (IF: 22.865) 链接:https://doi.org/10.1038/s41593-020-00736-x 作者使用单细胞转录组测序共捕获了 9141 个细胞,保留 4892 个高质量的肠神经元进行后续分析...
A total of 3,574 ± 2,010 distinct genes were identified in each cell (Usoskin et al., 2015), as compared with our study of 1,788 ± 283 genes per cell. A subsequent publication used neuron-size-based hierarchical clustering and high-coverage scRNA-seq of 203 neurons collected from ...
Population-scale single-cell RNA-seq profiling across dopaminergic neuron differentiation Jupyter Notebook216 Repositories XClonePublic Detection of allele-specific subclonal copy number alterations from single-cell transcriptomic data. Python29Apache-2.035(2 issues need help)0UpdatedAug 23, 2024 ...
They identified a new set of neurons in the human frontal cortex and redefine the neuron type based on the methylation group of neurons. Lake et al. [40] used a new single-cell nuclear sequencing method to map the second generation single cell of an adult brain. A variety of different ...
of single-cell RNA-seq Cell Stem Cell,Volume18 Supplemental Information Expression Analysis Highlights AXL as a Candidate Zika Virus Entry Receptor in Neural Stem Cells Tomasz J.Nowakowski,Alex A.Pollen,Elizabeth Di Lullo,Carmen Sandoval-Espinosa,Marina Bershteyn,and Arnold R.Kriegstein ...
In this study, we used single-cell RNA-sequencing (scRNAseq) to define the molecular logic by which SGN subtypes diversify in the mouse. Our detailed analysis of transcriptional dynamics of SGNs from several embryonic and perinatal stages indicates that neuronal subtypes successively emerge during HC...