we used the scRNA-seq data to derive gene signatures for each of the six malignant cell types,设计这些特征以平均权衡每种恶性细胞类型,并排除在AML细胞中普遍存在的正常细胞类型中表达的基因,从而将我们的方法与以前的研究(通过可变基因或分类人群的特征将AML分层)区别开来。We used our signatures to score...
RNA FISH (Fluorescence in situ hybridization) 技术是一种重要的非放射性原位杂交技术。 金开瑞利用荧光预标记的短寡核苷酸(长度约20个核苷酸),合并组成探针组(一组可多达48个独立探针)。将探针组与同一RNA靶标结合,即可产生不需要酶反应的信号扩增的强荧光信号,显示RNA或单RNA分子簇的点状斑点。由于阳性信号是由多...
Fig. 1: Single-cell RNA-seq reveals heterogeneity of normal scar and fibrotic skin disease dermis tissues. Dermal fibroblasts subcluster into distinct cell populations and mesenchymal fibroblasts are increased in fibrotic skin disease dermis Fig. 2: Keloid and normal scar fibroblasts subcluster into ...
Here, we used single-cell RNA sequencing (scRNA-seq) to decipher the composition and functions of GAMs in murine experimental GL261 gliomas grown in male and female mice. We demonstrate distinct transcriptional programs of microglia (MG), monocytes/macrophages (Mo/MΦ), and CNS BAMs. The iden...
Thus, scRNA-seq of normal BM reveals diverse hematopoietic cell types and implies differentiation trajectories consistent with current views of hematopoiesis. Single-Cell Profiling of AML Tumor Ecosystems To examine cellular diversity in AMLs, we obtained 35 cryopreserved BM aspirates from 16 AML ...
scRNA-seq: Single-cell RNA sequencing JEV: Japanese encephalitis virus CNS: Central nervous system DMEM: Dulbecco’s modified Eagle’s medium dpi: Days post infection IHC: Immunohistochemistry FISH: Fluorescence in situ hybridization UMI: Unique molecular identifier ...
1. Hedlund E, Deng Q. Single-cell RNA sequencing: Technical advancements and biological applications[J]. Molecular Aspects of Medicine, 2017, 59:36-46. 2. Sasagawa Y , Nikaido I , Hayashi T , et al. Quartz-Seq: A highly reproducible and sensitive single-cell RNA-Seq reveals non-genetic...
In summary, based on an integration of the single-cell RNA-seq results, the team demonstrated changes in the immune cell landscape of five different autoimmune diseases with respect to immune cell subsets, populations, differentially-expressed genes, and the cell-to-cell communication network. The ...
Myasthenia gravis (MG) is a rare autoimmune disease. Although the impact of immune cell disorder in MG has been extensively studied, little is known about the transcriptomes of individual cells. Here, we assessed the transcriptional profiles of 39,243 ce
bulk analyses of pre-selected cell types provide a view of the averaged global gene expression patterns from the cell population within the tissue type but do not differentiate the cell types in the sample. Single-cell RNA-sequencing (scRNA-seq) has revolutionized our ability to comprehensively ...