这些整合的蛋白质组学和基因组平台确定了RNA疫苗免疫的抗原特异性细胞基础。 6 英文题目:Single-cell transcriptomics and surface epitope detection in human brain epileptic lesions identifies pro-inflammatory signaling 中文题目:人脑癫痫病变中的单细胞转录组学和表面表位检测确定促炎信号 发表时间:2022-06-23...
scRNA-seq, single-cell RNA sequencing; TME, tumor immune environment; PDAC, pancreatic ductal adenocarcinoma; UMAP, uniform manifold approximation and projection; GSEA, gene set enrichment analysis; TMGs, T cell marker genes; TCGA; The Cancer Genome Atlas; TMGS, T cell marker genes score; Tcm...
To resolve the molecular profiles of CD11b+cells, we performed scRNA-seq. After quality control and adjusting for technical noise, single-cell transcriptomic profiles for 40,401 cells and 14,618 genes were selected for the analysis (see “Methods” section). We visually inspected the transcriptomi...
We performed single-cell RNA-Seq (scRNA-Seq) on tumours and matched lung macrometastases of patient-derived xenografts of breast cancer. After correcting for confounding factors such as the cell cycle and the percentage of detected genes (PDG), we identified cells in three states in both ...
The advent of single-cell RNA sequencing (scRNA-seq) has provided insight into the tumour immune microenvironment (TIME). This review focuses on the application of scRNA-seq in investigation of the TIME. Over time, scRNA-seq methods have evolved, and components of the TIME have been deciphered...
cell resolution, reveals the determinants of intratumor gene expression heterogeneity, and identifies the molecular bases for the formation of many oncological diseases [15]. Here, we describe the current state of and advances in scRNA-seq technology, summarize its applications in cancer biology ...
Here, we analyzed a single cell RNA sequencing (scRNA-seq) dataset during Atoh1-induced supporting cell (SC) to hair cell (HC) conversion in adult mouse cochleae (Yamashita et al. (2018)) using multiple high-throughput sequencing analytical tools (WGCNA, SCENIC, A...
Single-cell RNA sequencing (scRNA-seq) is a powerful tool for defining cellular diversity in tumors, but its application toward dissecting mechanisms underlying immune-modulating therapies is scarce. We performed scRNA-seq analyses on immune and stromal populations from colorectal cancer patients, identif...
In recent years, single-cell RNA sequencing (scRNA-seq) technology has revolutionized cancer metastasis research by providing unparalleled insights into the complex cellular heterogeneity [5, 6]. Several scRNA-seq studies related to breast cancer metastasis have been largely focused on cells in the tu...
To find candidate enhancers, single-cell analysis of accessible chromatin (scATAC-seq) data is preprocessed using pycisTopic, a faster Python reimplementation of cisTopic16 (Fig. 1b and Extended Data Fig. 1a–f). SCENIC+ uses both differentially accessible regions (DARs) and topics, sets of co...