这些整合的蛋白质组学和基因组平台确定了RNA疫苗免疫的抗原特异性细胞基础。 6 英文题目:Single-cell transcriptomics and surface epitope detection in human brain epileptic lesions identifies pro-inflammatory signaling 中文题目:人脑癫痫病变中的单细胞转录组学
scRNA-seq, single-cell RNA sequencing; TME, tumor immune environment; PDAC, pancreatic ductal adenocarcinoma; UMAP, uniform manifold approximation and projection; GSEA, gene set enrichment analysis; TMGs, T cell marker genes; TCGA; The Cancer Genome Atlas; TMGS, T cell marker genes score; Tcm...
To resolve the molecular profiles of CD11b+cells, we performed scRNA-seq. After quality control and adjusting for technical noise, single-cell transcriptomic profiles for 40,401 cells and 14,618 genes were selected for the analysis (see “Methods” section). We visually inspected the transcriptomi...
Single-cell RNA sequencing (scRNA-seq) is a powerful tool for defining cellular diversity in tumors, but its application toward dissecting mechanisms underlying immune-modulating therapies is scarce. We performed scRNA-seq analyses on immune and stromal populations from colorectal cancer patients, identif...
cell RNA-seq as a tool for identification of chicken peripheral blood leukocytes. For this purpose, purified and thrombocyte depleted leukocytes from 4 clinically healthy hens were subjected to single-cell 3′ RNA-seq. Bioinformatic analysis of data comprised unsupervised clustering of the cells, ...
In a human menstrual cycle the endometrium undergoes remodeling, shedding and regeneration, all of which are driven by substantial gene expression changes in the underlying cellular hierarchy. Despite its importance in human fertility and regenerative bi
Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity. Nat Commun. 2018;9:2028. Article PubMed PubMed Central CAS Google Scholar Dong J, Hu Y, Fan X, Wu X, Mao Y, Hu B, Guo H, Wen L, Tang F. Single-cell RNA-seq analysis unveils a ...
Here, we analyzed a single cell RNA sequencing (scRNA-seq) dataset during Atoh1-induced supporting cell (SC) to hair cell (HC) conversion in adult mouse cochleae (Yamashita et al. (2018)) using multiple high-throughput sequencing analytical tools (WGCNA, SCENIC, ARACNE, and VIPER). Instead...
The advancement of high-throughput single-cell RNA sequencing (scRNA-seq) technologies provides valuable insights into the diversity of cellular states and unravels their dynamic connections [1]. Systematically executing multiple single-cell data analysis tasks, such as functional gene modules detection, ...
, as each cell type may respond differently owing to its unique characteristics (specificity, immunological memory, and self/non-self-recognition). For the first time, we assessed the transcriptional response of E171 TiO2in rPBMC at the single-cell level using high-dimensional scRNA-seq analysis...