As a first step, asingle‐cell suspension is generatedin a process called single‐cell dissociation in which the tissue is digested. plate-based & droplet-based 但是都存在问题,In both cases, errors can occur that lead to multiple cells being captured together(doublets or multiplets),non‐viable...
Current best practices in single‐cell RNA‐seq analysis: a tutorialwww.embopress.org/doi/full/10.15252/msb.20188746 单细胞RNA测序(scRNA-seq )是一个飞速发展的领域,该技术帮助科研人员从一堆细胞(bulk)水平精细到单细胞(single-cell)水平研究基因的表达状态,从而可以更加精细的刻画科研对象的分子状态(例...
第二种方法是利用无监督的聚类方法先将不同的细胞类型聚类,然后连接每一个cell cluster,再把这些cell cluster投影到每一个分支上 还有一种是利用RNA降解速率来推断细胞发育轨迹,RNAvelocity就是利用这个原理,其原理大致为传送门 差异表达 在单细胞差异表达显著性检验中,往往利用非参数non-parametric Wilcoxon test做检验。
文献阅读-《Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data》 文献名称:教程:单细胞 RNA 测序数据的计算分析指南 文献期刊:nature protocols 发表时间:2021-1-9 摘要 单细胞 RNA 测序技术 (scRNA-seq) 是一种流行且功能强大的技术,可以分析大量单个细胞的整个转录表达谱。...
analysis pipeline development, computational biology, data analysis tutorial, single‐cell RNA‐seqSingle‐cell RNA‐seq has enabled gene expression to be studied at an unprecedented resolution. The promise of this technology is attracting a growing user base for single‐cell analysis methods. As more...
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows you to profile the whole transcriptome of a large number of individual cells. However, the analysis of the large volumes of data generated from these experiments requires specialized statistical and computational ...
J. Current best practices in single-cell RNA-seq analysis: a tutorial. Mol. Syst. Biol. 15, e8746 (2019). This scRNA-seq-focused review and workflow template served as inspiration for the current benchmark-based Expert Recommendation. Article PubMed Central PubMed Google Scholar Kharchenko,...
cell RNA-Seq using Seurat _ 33:38 8 Pseudo-bulk analysis for single-cell RNA-Seq data _ Detailed workflow tutorial 35:53 9 The Beginner's guide to bulk RNA sequencing vs single-cell RNA Sequencing 12:24 10 Single-cell Trajectory analysis using Monocle3 and Seurat _ Step-by-step tuto ...
Single‐cell RNA‐seq has enabled gene expression to be studied at an unprecedented resolution. The promise of this technology is attracting a growing user base for single‐cell analysis methods. As more analysis tools are becoming available, it is becoming increasingly ...
Defining cell types and states with single-cell genomics. Genome Res. 25, 1491–1498 (2015). Article CAS PubMed PubMed Central Google Scholar Luecken, M. D. & Theis, F. J. Current best practices in single-cell RNA-seq analysis: a tutorial. Mol. Syst. Biol. 15, e8746 (2019). ...