RNA FISH (Fluorescence in situ hybridization) 技术是一种重要的非放射性原位杂交技术。 金开瑞利用荧光预标记的短寡核苷酸(长度约20个核苷酸),合并组成探针组(一组可多达48个独立探针)。将探针组与同一RNA靶标结合,即可产生不需要酶反应的信号扩增的强荧光信号,显示RNA或单RNA分子簇的点状斑点。由于阳性信号是由多...
Advances in single-cell RNA sequencing (scRNA-seq) have allowed for comprehensive analysis of the immune system. In this Review, we briefly describe the available scRNA-seq technologies together with their corresponding strengths and weaknesses. We discuss in depth how scRNA-seq can be used to de...
Single-cell RNA-seq identifies myeloid cells with distinct expression profiles among CD11b+cells from naive and glioma-bearing brains We employed a murine orthotopic GL261 glioma model, because tumors established from GL261 cells recapitulate many characteristics of human GBMs and are frequently used ...
we used the scRNA-seq data to derive gene signatures for each of the six malignant cell types,设计这些特征以平均权衡每种恶性细胞类型,并排除在AML细胞中普遍存在的正常细胞类型中表达的基因,从而将我们的方法与以前的研究(通过可变基因或分类人群的特征将AML分层)区别开来。We used our signatures to score ...
批量RNA-seq分析提取样本中的所有RNA，可能包含数万或数十万个细胞，然后对RNA进行序列分析。然而，这种...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
【生信技术】测序数据差异表达分析|导入、加载、整理、分析,RNA-Seq数据的差异分析操作,跟着操作你就对了 231 -- 33:57 App Integrate single-cell RNA-Seq datasets in R using Seurat (CCA) 575 -- 12:56 App (字幕)Single Cell RNA Seqencing(scRNAseq)原理 - Finding a cure for DIPG 1028 -- 9:...
从这个系列开始，我们将跟着一篇《Cell》文章学习单细胞转录组数据的下游分析，以及下游分析数据的个性化...
在本周一的文章(复现原文(一):Single-cell RNA sequencing of human kidney(step by step))中,我们完成了scRNA-seq数据的质控(Hemberg-lab单细胞转录组数据分析(三)- 原始数据质控)、批次校正、找Marker基因(单细胞分群后,怎么找到Marker基因定义每一类群?)、UMAP可视化和tSNE可视化(Hemberg-lab单细胞转录组数据分...
by using single-cell RNA-seq. Our results indicate that keloid fibroblasts can be divided into 4 subpopulations: secretory-papillary, secretory-reticular, mesenchymal and pro-inflammatory. Interestingly, the percentage of mesenchymal fibroblast subpopulation is significantly increased in keloid compared to ...