Single-cell RNA-seq with waterfall reveals molecular cascades underlying adult neurogenesis. Cell Stem Cell 17, 360–372 (2015) Article CAS PubMed PubMed Central Google Scholar Macosko, E. Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. ...
Fig. 1: High-quality profiling of new RNAs in single cells with NASC-seq2. a, Plot showing the number of genes detected per K562 cell as a function of reads sequenced, for K562 cells processed with NASC-seq2 (613 individual cells) and NASC-seq20 (138 individual cells), respectively. ...
The Microwell-seq developed by Han et al. is a high-throughput and low-cost scRNA-seq platform [8] (Table 1). Not only does it improve the detection abundance of single-cell technologies, but it also reduces the cost of detection by an order of magnitude compared to single-cell ...
We integrated these data in a machine learning classifier that distinguished malignant from normal cells and identified six malignant AML cell types that project along the HSC to myeloid differentiation axis. We use this resource to relate developmental hierarchies to genotypes, to evaluate properties ...
The tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) is a complex ecosystem that drives tumor progression; however, in-depth single cell characterization of the PDAC TME and its role in response to therapy is lacking. Here, we perform single-cell RNA sequencing on freshly ...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
RNA from each cell was isolated and used to construct a single mRNA-seq library per cell, which was then sequenced using Hi-seq. (B) t-SNE plot of the RNA-seq data from 363 EGFPlow and EGFPhigh cells. (C) The same t-SNE plot (as shown in B) but with points colored based on ...
Drawing the Developmental Landscape of the Human Prefrontal Cortex by Single-cell RNAseq The prefrontal cortex(PFC)is the cerebral cortex which covers the front part of the frontal lobe in mammalian brain anatomy and in the case of ... WANG,Xiaoqun - 《Bulletin of the Chinese Academy of Scien...
Moreover, we found that A-to-I editings in miRNA-targeted regions of a substantial number of genes preferably occurred in one or two sequential stages. Conclusions Our single-cell analysis reveals dynamic changes in genome-wide RNA-editing during early human embryogenesis in a stage-specific ...
To accomplish this, we collated six single cell datasets comprising different modalities and existing ground truth labels: two scRNASeq datasets from breast35 and lung36 cancer cell lines where each cell line forms a “cell type” to predict, a pancreatic cancer single nucleus RNA Sequencing (sn...