SingleR - Single-cell Recognition Recent advances in single cell RNA-seq (scRNA-seq) have enabled an unprecedented level of granularity in characterizing gene expression changes in disease models. Multiple single cell analysis methodologies have been developed to detect gene expression changes and to cl...
the signal-to-noise ratio in our experiments examining Blimp1-dependent transcripts in the placenta was substantially dampened by contributions from Blimp1-independent cell types. Recent advances in RNA-seq technology
We classified scRNA-seq data into 34 cell types based on their gene expression profiles22and examined AS in 21 peripheral blood mononuclear cell (PBMC) subtypes at two levels (Methods, Fig.1eand Extended Data Fig.1c). At the single-cell level, SpliZ23detected a median of 1,146 AS genes...
To comprehensively delineate the complex intra-tumoral heterogeneity and the underlying mechanism during metastatic lesions malignant progression, single-cell RNA sequencing (scRNA-seq) was employed. PCA and TSNE were used for dimensionality reduction analysis and cell clustering. Find All Markers function ...
Single-cell RNA sequencing is a powerful technique that advances gene expression regulation research to a higher resolution level for single cells. Recent advancements in scRNA-Seq methods (e.g., Droplet-based) allow thousands of cells to be captured and sequenced in a relatively short time frame...
Single-cell RNA-sequencing (scRNA-seq) technology has created unprecedented opportunities to simultaneously assess thousands of cells within a sample, enabling the evaluation of heterogeneity among tumor cells17,18. Furthermore, scRNA-seq provides unique opportunities to assess the regulation, evolution ...
Single-Cell RNA-Seq of Neural Precursor Cells from the Adult Mouse Dentate Gyrus In the adult dentate gyrus, radial-glia-like qNSCs give rise to new neurons via a sequential process of activation, proliferation, and generation of intermediate precursor cells (IPCs; Figure 1A) (Ming and Song,...
et al. CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq. Genome Biol. https://doi.org/10.1186/s13059-016-0938-8 (2016). Article PubMed PubMed Central Google Scholar Tang, F. et al. mRNA-Seq whole-transcriptome analysis of a single cell. Nat. Methods https://doi.org/...
Rodents represent the main species for studies on the cellular and molecular basis of nociception and the greatest insights with respect to molecular classification of neuronal types have been obtained from mouse, where single-cell RNA-sequencing (scRNA-seq) has led to a molecular taxonomy of ...
2017年7月,奥地利研究人员在Nature Methods上发表“Pooled CRISPR screening with single-cell transcriptome readout (doi:10.1038/ nmeth.4227)”文章,创造性地将CRISPR-Cas9和单细胞RNA测序两种极有前景的基因组学领域结合在一起,发明了这种被称作CROP-seq的单细胞测序方法 (CRISPR droplet sequencing, CRISPR液滴测...