Single-cell RNA sequencing (scRNA-seq) allows the comprehensively profiling of the gene expression changes observed in development at a cellular level [19], while single-cell transposase-accessible chromatin sequencing (scATAC-seq) allows the analysis of chromatin accessibility and transcription factor (...
We classified scRNA-seq data into 34 cell types based on their gene expression profiles22and examined AS in 21 peripheral blood mononuclear cell (PBMC) subtypes at two levels (Methods, Fig.1eand Extended Data Fig.1c). At the single-cell level, SpliZ23detected a median of 1,146 AS genes...
To comprehensively delineate the complex intra-tumoral heterogeneity and the underlying mechanism during metastatic lesions malignant progression, single-cell RNA sequencing (scRNA-seq) was employed. PCA and TSNE were used for dimensionality reduction analysis and cell clustering. Find All Markers function ...
Single-cell RNA-seq identifies discrete Prdm1 þ cell types. Hierarchical clustering of the data revealed two distinct groups, each containing three clear subdivisions designated 1A–C and 2A–C (Fig. 2a). Principle component analysis (PCA) using all 15,402 detected genes revealed the same ...
Single-Cell RNA-Seq of Neural Precursor Cells from the Adult Mouse Dentate Gyrus In the adult dentate gyrus, radial-glia-like qNSCs give rise to new neurons via a sequential process of activation, proliferation, and generation of intermediate precursor cells (IPCs; Figure 1A) (Ming and Song,...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
and each edge corresponds to a regulatory relationship that has been documented in biological databases. We train the KPNNs based on single-cell RNA-seq data for the cell type or biological model of interest, using an optimized learning method that enhances the biological interpretability of the ...
2017年7月,奥地利研究人员在Nature Methods上发表“Pooled CRISPR screening with single-cell transcriptome readout (doi:10.1038/ nmeth.4227)”文章,创造性地将CRISPR-Cas9和单细胞RNA测序两种极有前景的基因组学领域结合在一起,发明了这种被称作CROP-seq的单细胞测序方法 (CRISPR droplet sequencing, CRISPR液滴测...
Rodents represent the main species for studies on the cellular and molecular basis of nociception and the greatest insights with respect to molecular classification of neuronal types have been obtained from mouse, where single-cell RNA-sequencing (scRNA-seq) has led to a molecular taxonomy of ...
SingleR - Single-cell Recognition Install Updates Usage Contributors Recent advances in single cell RNA-seq (scRNA-seq) have enabled an unprecedented level of granularity in characterizing gene expression changes in disease models. Multiple single cell analysis methodologies have been developed to detect ...