Cell-Type-Specific RegulonAlzheimer's disease (AD) is a progressive neurodegenerative disorder of the brain and the most common form of dementia among the elderly. The single-cell RNA-sequencing (scRNA-Seq) and single-nucleus RNA-sequencing (snRNA-Seq) techniques are extremely useful for ...
确定一下 cell 名的组成,构建好 metadata(行名) 和 count(列名) 矩阵能够对应 过滤掉外源 RNA (ERCC) 合并成为 seurat 对象 计算线粒体(MT),核糖体的 RNA(^RP[SL]) 比例并筛选数据 我们先处理第一个表达矩阵 # 这个是治疗前的第一个表达矩阵 # 导入数据 raw.data = read.csv('csv_files/S01_datafin...
Alzheimer’s Disease (AD) pathology has been increasingly explored through single-cell and single-nucleus RNA-sequencing (scRNA-seq & snRNA-seq) and spatial transcriptomics (ST). However, the surge in data demands a comprehensive, user-friendly repos
Single-cell RNA sequencing (scRNA-Seq) experiments are gaining ground to study the molecular processes that drive normal development as well as the onset of different pathologies. Finding an effective and efficient low-dimensional representation of the data is one of the most important steps in the...
针对于初入门者的single-cell RNA-seq数据分析在线课程资料: Analysis of single cell RNA-seq datahemberg-lab.github.io/scRNA.seq.course/ 主讲人Vladimir Kiselev主编SC3和scmap两个single-cell RNA-seq数据分析包,其导师Martin Hemberg是剑桥教授。 有意思的是,现在R里处理single-cell RNA sequencing的package...
The single-cell RNA-sequencing (scRNA-Seq) and single-nucleus RNA-sequencing (snRNA-Seq) techniques are extremely useful for dissecting the function/dysfunction of highly heterogeneous cells in the brain at the single-cell level, and the corresponding data analyses can significantly improve our ...
The count table, a numeric matrix of genes × cells, is the basic input data structure in the analysis of single-cell RNA-sequencing data. A common preprocessing step is to adjust the counts for variable sampling efficiency and to transform them so
单细胞RNA测序Single Cell RNA Sequencing vs. Bulk RNA Sequencing 3876 1 7:35:42 App 生肉|Single cell RNA-seq data analysis with R (2019) 单细胞RNA测序数据分析教程 2万 31 31:32 App 30分钟拿下RNA-seq原理、操作方法、分析方法和应用场景 443 -- 3:15:30 App 单细胞RNA-seq ChiPster 6599...
database example man vignettes .Rbuildignore .gitattributes DESCRIPTION NAMESPACE README.html README.md scMLnet_0.1.0.tar.gz README scMLnet Introduction scMLnet is an R package developed to construct inter-/intracellular multilayer singaling network based on single-cell RNA-seq expression data. sc...
2.SCONE:single-cell RNA-seq data 质检和标准化的包 3.Seurat,R包 4.ASAP:(Automated Single-cell Analysis Pipeline,一个网络平台 而关于单细胞RNA-seq的局限主要来自: 1.扩增,每个cell分得的reads 2.细胞基因的特异性表达,以区分不同类的细胞