single-celltranscriptomestem cellsheterogeneitytestisMammalian spermatogenesis is a complex developmental program that transforms mitotic testicular germ cells (spermatogonia) into mature male gametes (sperm) for production of offspring. For decades, it has been known that this several-weeks-long process ...
RNA-seq harnesses next-generation sequencing (NGS) to ascertain and quantify RNA sequence reads within a sample. Its prime objective is to furnish insights regarding gene expression, with particular emphasis on: Level of Expression: Through read counts, RNA-seq provides information regarding the abund...
Cell Ranger is a set of analysis pipelines that process Chromium single-cell RNA-seq output to align reads, generate feature-barcode matrices and perform clustering and gene expression analysis. Cell Ranger includes four pipelines relevant to single-cell gene expression experiments: cellranger mkfastq...
Meet Dr. Nicholas Navin and learn about his work on the Human Breast Cell Atlas — the world’s most comprehensive single-cell atlas of healthy breast tissue.
During this process, each unique DNA molecule in the library is bound to the surface of a bead or a flow-cell and PCR amplified to create a set of identical clones. In the case of Ion Torrent technology, a process called “templating” is...
Recently, there has been a growing interest in using single-cell technologies to identify novel biomarkers. Single-cell RNA sequencing (scRNA-seq) is a powerful tool for identifying gene transcripts within individual cells and can be used to detect changes in gene expression that may be indicative...
Single-cell RNAseq reveals seven classes of colonic sensory neuron. Gut 68, 633–644 (2018). This study identifies seven subtypes of sensory neurons innervating the colon of mice using single-cell RNA sequencing, therefore providing the molecular signature of specific neurons projecting to the ...
Single cell RNA-seq The development of high-throughput methods for transcriptomic analysis at the single-cell level, known as single-cell RNA-seq (scRNA-seq) (Fig.2f), has revolutionized our understanding of cell types and states in various biological processes. Within the technological framework ...
Patel AP, Tirosh I, Trombetta JJ, Shalek AK, Gillespie SM, Wakimoto H, Cahill DP, Nahed BV, Curry WT, Martuza RL, Louis DN, Rozenblatt-Rosen O, Suva ML, Regev A, Bernstein BE (2014) Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma. Science 344:1396–14...
Single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to 2019-nCoV infection. Front. Med. 14, 185–192 (2020). Article PubMed Google Scholar Ren, X. et al. Multiple expression assessments of ACE2 and TMPRSS2 ...