single‐cell RNA sequencingT cellsThe use of singleヽell RNA sequencing (scRNA﹕eq) in microglial research is increasing rapidly. The basic workflow of this approach consists of isolating single cells, followed by sequencing. scRNA﹕eq is capable of examining microglial heterogeneity on a cellular ...
resulting in one library which is sequenced across multiple flow cells. This workflow is commonly performed to increase sequencing depth. In this case, all reads can be combined in a single instance of thecellranger countormultipipeline. This process is described in Specifying Input FASTQ pages...
What is RNA sequencing?RNA sequencing, or RNA-seq, is a powerful high-throughput, next generation sequencing (NGS) technique used to examine RNA in a single cell or a population of cells [1,2]. RNA-seq provides researchers with insights into gene expression levels, which allows them to ...
Prior to sequencing, the DNA library must be attached to a solid surface and clonally amplified to increase the signal that can be detected from each target during sequencing. During this process, each unique DNA molecule in the library...
Categories: NGS | Single-cell | Automation | Research News The use of next-generation sequencing for transcriptome analysis in clinical and applied spaces requires accurate, parallel processing of large numbers of single cells. Several platforms have emerged to meet this need but variations in the ...
Single-cell RNA sequencing (scRNA-seq) is a tool for studying gene expression at the single-cell level that has been widely used due to its unprecedented high resolution. In the present review, we outline the preparation process and sequencing platforms for the scRNA-seq analysis of solid tumor...
This method can accurately diagnose pan cancer microsatellite instability by single molecule reverse probe capture and high-throughput sequencing. According to this study, smMIPs can only accurately identify microsatellite instability in colorectal, prostate and endometrial cancers to determine the presence ...
He says that single-cell RNA sequencing creates a gene-centric view that distracts scientists from other questions. “I think a cell is much more than the sum total of the RNAs that it contains,” says Martinez Arias. For example, when he grows cells in...
Real-time PCR enables you to screen known SNPs. The benefits of real-time PCR are that it is easy, accurate, and can scale to high throughput. Another advantage of real-time PCR is that the bioinformatic analysis is less complex than for other t...
Single-cell RNAseq reveals seven classes of colonic sensory neuron. Gut 68, 633–644 (2018). This study identifies seven subtypes of sensory neurons innervating the colon of mice using single-cell RNA sequencing, therefore providing the molecular signature of specific neurons projecting to the ...