single‐cell RNA sequencingT cellsThe use of singleヽell RNA sequencing (scRNA﹕eq) in microglial research is increasing rapidly. The basic workflow of this approach consists of isolating single cells, followed by sequencing. scRNA﹕eq is capable of examining microglial heterogeneity on a cellular ...
In this example you have one sample that is processed through one GEM well then you generate one library which is sequenced across multiple flowcells. This may be done to increase sequencing depth, for example. In this case all of the reads can be combined in a single instance of thecell...
Prior to sequencing, the DNA library must be attached to a solid surface and clonally amplified to increase the signal that can be detected from each target during sequencing. During this process, each unique DNA ...
Prior to DNA methylation sequencing, researchers treat their samples with sodium bisulfite to distinguish methylated cytosine from unmethylated cytosine.
Fragment analysis is a genetic analysis method comprising a series of techniques in which DNA fragments are fluorescently labeled, separated by capillary electrophoresis (CE), and sized by comparison to an internal standard. CE-based genetic analyzers are capab...
This method can accurately diagnose pan cancer microsatellite instability by single molecule reverse probe capture and high-throughput sequencing. According to this study, smMIPs can only accurately identify microsatellite instability in colorectal, prostate and endometrial cancers to determine the presence ...
to form structures calledchromosomes. Each chromosome contains a single DNA molecule, wrapped tightly around spool-like proteins called histones, which provide chromosomes their structure. Humans have 23 pairs of chromosomes, which are found inside each cell's nucleus, the control center of the cell...
to form structures calledchromosomes. Each chromosome contains a single DNA molecule, wrapped tightly around spool-like proteins called histones, which provide chromosomes their structure. Humans have 23 pairs of chromosomes, which are found inside each cell's nucleus, the control center of the cell...
Real-time PCR enables you to screen known SNPs. The benefits of real-time PCR are that it is easy, accurate, and can scale to high throughput. Another advantage of real-time PCR is that the bioinformatic analysis is less complex than for other t...
Unlike tissue biopsy where the tumor DNA is preserved in formalin-fixed paraffin embedded (FFPE) blocks, DNA cross linking does not occur with liquid biopsy; thereby facilitating tumor DNA sequencing. Wafik El-Deiry MD, discusses the benefits that liquid biopsies can offer patients. ...