sales@vazyme.com 技术支持/support@vazyme.com 技术服务/service@vazyme.comVazyme Biotech Co., Ltd Web/www.vazyme.com Tel/400-600-9335 Sales/sales@vazyme.com Support/support@vazyme.com Service/service@vazyme.com Vazyme biotech co., ltd.P621-01Single Cell Sequence Specific Amplification Kit产品组成...
The total amount of raw sequence mapped to a reference genome, divided by the length of the genome. Whole-genome amplification (WGA). Refers to methods that are used to amplify the genomic DNA of single cells to increase the number of copies of DNA for downstream processing. Clonal expansion...
The single-cell sequencing technologies can sequence and quantify the whole genome of germ cells and embryonic cells at the single-cell level (Fig. 2). This will help to understand the occurrence of germ cells and the screening, diagnosis, and treatment of reproductive and genetic diseases. Germ...
4. Sasagawa Y , Danno H , Takada H , et al. Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads[J]. Genome Biology, 2018, 19(1):29. 5. Nakamura T , Yabuta Y , Okamoto I , et al. SC3-seq: a method for highly parallel ...
ref.31.c, Schematic describing the parameters used to train the random forest model. Reads spanning a stop codon were used for training. The model predicts the offset between the 5ʹ end of each read and the P-site based on the read length and the sequence context around each end of ...
鉴于在单细胞水平来做这件事情,这可能确实具有挑战性。由于转录爆发和其他来源的基因表达的随机变化,基因表达可能会与TF输入的动力学部分断开(不相关)。目前已经有一些从单细胞RNA-seq数据推断共表达网络的方法发表,但是,这些方法未使用调控序列(regulatory sequence)分析来预测TF与靶基因之间的相互作用。
表观基因组(Epigenome): 来自相同基因组序列的不同基因表达模式能够稳定传播的组合特征(The combined features that enable stable propagation of different gene expression patterns from the same genome sequence)。包括胞嘧啶碱基的DNA甲基化(mC)、组蛋白的化学修饰、染色质可及性和高级染色质结构。
SEquence Quality Control (SEQC -- /sek-si:/) Overview: SEQC is a python package that processes single-cell sequencing data in the cloud and analyzes it interactively on your local machine. To faciliate easy installation and use, we have made available Amazon Machine Images (AMIs) that come ...
最后作者总结道:This observation opens the possibility of recording endogenous or exogenous events by barcode editing;just as evolutionary history is recorded in genome sequence changes, a cell's history might be recorded by barcode sequence edits....
Over the past decade, it has become nearly routine to sequence genomes of individual microbial cells directly isolated from environmental samples ranging from deep-sea hydrothermal vents to insect guts, providing a powerful complement to shotgun metagenomics in microbial community studies. In this review...