R. Single-nuclei RNA sequencing assessment of the hepatic effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Cell Mol. Gastroenterol. Hepatol. 11, 147–159 (2021). Article PubMed Google Scholar Wolfien M., et al. Single-nucleus sequencing of an entire mammalian heart: cell type composition ...
Liu-Lin Xiong from the Affiliated Hospital of Zunyi Medical University, China, and coworkers used single-cell RNA sequencing to profile gene activity in individual peripheral blood mononuclear cells from people with and without Alzheimer’s disease. They discovered that people with Alzheimer’s, ...
Single-cell sequencing technologies have revealed a wealth of information regarding cellular heterogeneity in health and disease in multiple tissues [1,2,3,4,5,6,7,8]. In particular, single-cell RNA sequencing enables the identification of groups of cells in a population with similar transcriptomic...
cell transcriptome sequencing results. The use of degreasing reagents, such as Triton X-100, in the cell lysis process can effectively dissolve cells and remove lipids to ensure the acquisition of high-quality RNA [39,43]. Lastly, hepatocytes are particularly rich in mitochondria, leading to elev...
Single-nucleus RNA-seq of nearly 20,000 nuclei has been used to study different postnatal developmental stages of the mouse heart, which has revealed major and rare cardiac cell types as well as significant anatomical and functional heterogeneity among all cell types in the postnatal developing ...
Single-nuclei RNA sequencing assessment of the hepatic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin. Cell Mol Gastroenterol Hepatol. 2021;11:147–59. CAS PubMed Google Scholar Castell JV, Jover R, Martinez-Jimenez CP, Gomez-Lechon MJ. Hepatocyte cell lines: their use, scope and ...
[18]. Notably, snRNA-seq can avoid the cell dissociation step by using detergents to directly release nuclei from intact cells [18]. Instead of sequencing all the Mrna in the cytoplasm, snRNA-seq captures only the transcripts in the nucleus [70]. Thus, it is understandable that these ...
For each experiment, the purified liver nuclei of four mice were mixed 1:1:1:1 prior tagmentation. Sequencing metrics can be found in Supplementary Table 2, and the initial analysis and comparison of the two individual biological replicates using Signac33 can be found in Extended Data Fig. 4...
To elaborate, an integrated analysis of cancer cells has been shown in hepatocellular carcinoma (HCC) where time-of-flight mass cytometry (CyTOF) and single-cell RNA sequencing were used to show an immunosuppressive gradient of immune cells in a tumour microenvironment, non-tumour microenvironment an...
Communication between cells is essential in maintaining homeostasis. The persistent disruption of cell–cell communication by environmental contaminants contributes to progressive disease and toxicity. In this study, single-nuclei RNA sequencing (snRNAse