Here, we perform single-nucleus RNA-sequencing (snRNA-seq) on nearly 300,000 nuclei from the parietal cortex of AD autosomal dominant (APP and PSEN1) and risk-modifying variant (APOE, TREM2 and MS4A) carriers. Within individual cell types, we capture genes commonly dysregulated across variant...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
Defining cellular and molecular identities within the kidney is necessary to understand its organization and function in health and disease. Here we demonstrate a reproducible method with minimal artifacts for single-nucleus Droplet-based RNA sequencing
Bulk RNA sequencing (RNA-seq) has been used as a powerful tool for investigating these orthopedic disorders9because it can provide an overview of biological components, enable identification of new genes, and elucidate related signaling networks involved in disease processes. However, this method does...
Mature messenger RNA NIA-AA: National Institute on Aging-Alzheimer’s Association nUMI: Number of unique molecular identifiers OPC: Oligodendrocyte precursor cells PC: Principal components pmi: Pointwise mutual information PMI: Postmortem interval pre-mRNA: Nuclear precursor mRNA PSEN1: Preseni...
Here, we used single-cell RNA sequencing (scRNA-seq) to decipher the composition and functions of GAMs in murine experimental GL261 gliomas grown in male and female mice. We demonstrate distinct transcriptional programs of microglia (MG), monocytes/macrophages (Mo/MΦ), and CNS BAMs. The ident...
the lower quantity of RNA per cell and smaller number of sampled cells in the single nuclear data provided only a higher-order perspective in the published analysis. Our higher-resolution model enables us to more precisely map the microglial subset implicated in AD and to guide the selection of...
Nuclear transcriptomes at high resolution using retooled INTACT Plant Physiol., 176 (2018), pp. 270-281 CrossrefView in ScopusGoogle Scholar Ryu et al., 2019 K.H. Ryu, L. Huang, H.M. Kang, J. Schiefelbein Single-cell RNA sequencing resolves molecular relationships among individual plant cel...
How to cite this article:Lacar, B.et al. Nuclear RNA-seq of single neurons reveals molecular signatures of activation.Nat. Commun.7:11022 doi: 10.1038/ncomms11022 (2016). Accession codes Accessions Gene Expression Omnibus GSE77067 In the original version of this Article, the middle names or ...
Nuclear RNA-seq of single neurons reveals molecular signatures of activation Single-cell sequencing methods have emerged as powerful tools for identification of heterogeneous cell types within defined brain regions. Application of single-cell techniques to study the transcriptome of activated neurons can ...