We provide an optimized single-nucleus RNA sequencing pipeline for clinical specimens with extensive quality assessments from tissue procurement through to single-cell transcriptomic analyses (Supplementary Fig.12). This tissue processing and interrogation work-flow is applicable to even limiting amounts of...
this software quantifies the expression for mature messenger RNA (mRNA) by counting reads aligned to exons as annotated in the human genome build GRCh38. However, the snuclRNA-seq profiles nuclear precursor mRNA (pre-mRNA), which includes transcripts that ...
Cryptic exon detection and transcriptomic changes revealed in single-nuclei RNA sequencing of C9ORF72 patients spanning the ALS-FTD spectrum The C9ORF72-linked diseases amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are characterized by the nuclear depletion and cytoplasmi... G...
the lower quantity of RNA per cell and smaller number of sampled cells in the single nuclear data provided only a higher-order perspective in the published analysis. Our higher-resolution model enables us to more precisely map the microglial subset implicated in AD and to guide the selection of...
Here, we used single-cell RNA sequencing (scRNA-seq) to decipher the composition and functions of GAMs in murine experimental GL261 gliomas grown in male and female mice. We demonstrate distinct transcriptional programs of microglia (MG), monocytes/macrophages (Mo/MΦ), and CNS BAMs. The ident...
How to cite this article:Lacar, B.et al. Nuclear RNA-seq of single neurons reveals molecular signatures of activation.Nat. Commun.7:11022 doi: 10.1038/ncomms11022 (2016). Accession codes Accessions Gene Expression Omnibus GSE77067 In the original version of this Article, the middle names or ...
Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression of chronic diseases. In order to expand our knowledge on cellular heterogeneity, we have developed a single-nucleus RNA-seq2 method tailored for the comprehensive analysis of the nuclear transcriptome fr...
Here, we show by single nuclear RNA-sequencing of cardiomyocytes from both mouse and human failing, and non-failing adult hearts that sub-populations of cardiomyocytes upregulate cell cycle activators and inhibitors consequent to the stress-response in vivo. We characterize these subgroups by weighted...
Here, we present a single-cell RNA sequencing atlas of immune cells from cartilaginous (white-spotted bamboo shark) and bony (zebrafish and Chinese tongue sole) fishes. Cross-species comparisons show that the same cell types across different species exhibit similar transcriptional profiles. In the ...
Lung adenocarcinoma (LUAD) harboring EGFR mutations prevails in Asian population. However, the inter-patient and intra-tumor heterogeneity has not been addressed at single-cell resolution. Here we performed single-cell RNA sequencing (scRNA-seq) of total