we performed single-nuclei RNA sequencing in a Nurr1 LV-shRNA mouse model.The results revealed cellular heterogeneity in the substantia nigra and a number of activated genes,the preponderance of which encode components of the major histocompatibility Ⅱ complex.Cd74,H2-Ab1,H2-Aα,H2-Eb1,Lyz2...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
We generated unsorted single-nuclei RNA sequencing data from brain tissue. We leveraged the tissue donated from a carrier of a Mendelian genetic mutation,PSEN1 p.A79V, and two family members who suffer from sporadic AD, but do not carry any autosomal mutations. We evaluated alternative alignment ...
Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zachary,Dalia,BarayanSarah,RehouAbdikarim,AbdullahiLauar,de Brito MonteiroMarc G.,Jeschke - 《Annals of Surgery》 被引...
(n = 6), one from the macula region and the other from the peripheral region, were collected and subjected to single-cell nuclei RNA-Seq. After dispensing, nanowells were imaged and only wells with single nuclei were selected. cDNA library construction and sequencing were performed for a...
Single nuclei RNA sequencing (snRNA-seq) has evolved as a powerful tool to study complex human diseases. Single cell resolution enables the study of novel cell types, biological processes, cell trajectories, and cell–cell signaling pathways. snRNA-seq largely relies on the dissociation of intact ...
sequencingthetranscriptomeofacellnucleus.Nucleiareisolated fromspecimensandsortedbyFACS,cDNAlibrariesareconstructedandRNA-seqisperformed, followedbydataanalysis.Somestepsfollowpublishedmethods(Smart-seq2forcDNAsynthesis andNexteraXTbarcodedlibrarypreparation)andarenotdescribedindetailhere.Previoussingle- cellapproaches...
Bulk brain tissue cell-type deconvolution with bias correction for single-nuclei RNA sequencing data using DeTREM O’Neill NK, Stein TD, Hu J, Rehman H, Campbell JD, Yajima M, Zhang X, Farrer LA BMC Bioinformatics. 2023 Sep 19https://doi.org/10.1186/s12859-023-05476-w ...
Single Nuclei RNA Sequencing analysis of MND muscle cultures for the paper "Impaired signaling for neuromuscular synaptic maintenance is a feature of Motor Neuron Disease" by Ding et al, published in Acta Neuropathologica Communications. r research cell scrna-seq muscle single-cell research-paper sin...