Here, we apply single nuclear RNA sequencing to compare cirrhotic livers from patients with and without PoPH. We identify characteristics unique to PoPH in cells surrounding the central hepatic vein, including increased growth differentiation factor signaling, enrichment of the a...
单细胞核转录组测序(singlecellnuclearRNASequencing)可以测试具体哪些项呀? 单细胞核转录组测序(singlecellnuclearRNASequencing)具备哪些检测资质呢? 单细胞核转录组测序(singlecellnuclearRNASequencing)如何收费呀? 单细胞核转录组测序(singlecellnuclearRNASequencing)参考哪些检测标准啊? 单细胞核转录组测序(singlecellnucl...
RNA-sequencing (snRNA-seq) methods, here we show that snRNA-seq faithfully recapitulates transcriptional patterns associated with experience-driven induction of activity, including immediate early genes (IEGs) such asFos,ArcandEgr1. SnRNA-seq of mouse dentate granule cells reveals large-scale changes ...
we show by single nuclear RNA-sequencing of cardiomyocytes from both mouse and human failing, and non-failing adult hearts that sub-populations of cardiomyocytes upregulate cell cycle activators and inhibitors consequent to the stress-response in vivo. We characterize these subgroups by weighted gene ...
RNA sequencing displayed >3 fold upregulation of CHRNA1 (P< 0.001) after injury alone and >18 fold upregulation 7 days following reconstruction surgery (P< 0.001). This was accompanied by a host of other neuromuscular regulatory genes including HDAC4 (P< 0.001), GDNF (P< 0.001), and ...
Nuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries. However, certain deficiencies severely restrict
and found that the numbers of Unigenes vary substantially from gene copy numbers from the genome sequences - most likely resulting from sequencing error, assembly errors, number of ESTs, alternative splicing, allelism and other such factors. Using a set of criteria (see Methods) designed to maxi...
RNA ofNyctotherus ovaliswith the "cDNA library Construction" kit from BD Biosciences/Clontech according prescription. The cDNA was ligated in the vector pDNR-LIB and transformed inE. coliElectromax DH10B cells (Invitrogen). Titer: 2.58.107. Randomly picked clones were selected for sequencing. ...
suggestingthattheyareofancientorigin.RNA-blothybridisationshowedthatthegenewasexpressedinshoots,butnotroots,andwaslightregulatedandrepressedbysucrose.Theexpressionofchi-mericgenesconsistingofPetCpromoterfragmentsfusedtothe>>>-glucuronidase(GUS)reportergenewasexaminedinA.thalianaandtobacco.InA.thaliana,GUSactivitywasdete...
we performed single-nuclei RNA sequencing in a Nurr1 LV-shRNA mouse model.The results revealed cellular heterogeneity in the substantia nigra and a number of activated genes,the preponderance of which encode components of the major histocompatibility Ⅱ complex.Cd74,H2-Ab1,H2-Aα,H2-Eb1,Lyz2...