1a). For congruence with the original nascent RNA sequencing method of GRO–seq, we named this single-cell version scGRO–seq. Intact nuclei containing nascent RNA labelled with propargyl, following a nuclear run-on reaction with 3′-(O-propargyl)-NTPs, were sorted individually into 96-well ...
Analyses of transcriptional bursting from single-cell RNA-sequencing data have revealed patterns of variation and regulation in the kinetic parameters that could be inferred. Here we profiled newly transcribed (4-thiouridine-labelled) RNA across 10,000 individual primary mouse fibroblasts to more broadly...
RNA sequencing of single cells (scRNA-seq) enables the global gene expression patterns of individual cells to be defined. Almost all tissues and organs include a heterogeneous mix of cell types; the heterogeneity of these cell populations can be defined through the use of scRNA-seq. scRNA-seq ...
11,12]. For example, Kirita et al. [13] identified distinct pro-inflammatory and pro-fibrotic proximal tubular cells (FR-PTCs) using single nuclear RNA sequencing (snRNA-seq) kidneys of ischemia reperfusion injury (IRI) -AKI
cell and single-nuclei RNA-sequencing (snRNA-seq) methods, here we show that snRNA-seq faithfully recapitulates transcriptional patterns associated with experience-driven induction of activity, including immediate early genes (IEGs) such asFos,ArcandEgr1. SnRNA-seq of mouse dentate granule cells ...
[12] combined sNuc-Seq with microfluidic technology to introduce a single-cell nuclear RNA sequencing method with low cost, high-sensitive and high-efficiency cell classification, which is expected to be used in human cell mapping projects (Table 1). Lake et al. [13] combined single-core ...
pathological traits of AD. Following image acquisition with a fluorescence microscope, automated image analysis was done using CellProfiler.cIndependent replication of the basic population structure of microglia. We used a recently published human microglia single-cell RNA sequencing dataset to confirm the...
cell RNA sequencing (scRNA-seq) of CD11b+myeloid cells in naïve and GL261 glioma-bearing mice that reveal distinct profiles of microglia, infiltrating monocytes/macrophages and CNS border-associated macrophages. We demonstrate an unforeseen molecular heterogeneity among myeloid cells in naïve and ...
We present a snRNASeq protocol and compare its results with scRNA-seq. Two nuclear suspensions were prepared in lysis buffer on ice while one cell suspension was generated using enzymatic and mechanical dissociation. Cells and nuclei were processed using the 10x Genomics platform, and sequencing ...
Advances in single-cell RNA sequencing (scRNA-seq) technologies in the past 10 years have had a transformative effect on biomedical research, enabling the profiling and analysis of the transcriptomes of single cells at unprecedented resolution and throughput. Specifically, scRNA-seq has facilitated the...