inflammation, neuronal cell death, and white matter injury9,10. This tissue damage is met with pronounced transcriptional, biochemical, and morphological changes in glial cells, including reactive astrogliosis and early remyelination10,11. However, current knowledge on the phenotypic heterogeneity within ...
However, the cell-specific effect of variants in these genes is not fully understood. Here, we perform single-nucleus RNA-sequencing (snRNA-seq) on nearly 300,000 nuclei from the parietal cortex of AD autosomal dominant (APP and PSEN1) and risk-modifying variant (APOE, TREM2 and MS4A) ...
Analyses of transcriptional bursting from single-cell RNA-sequencing data have revealed patterns of variation and regulation in the kinetic parameters that could be inferred. Here we profiled newly transcribed (4-thiouridine-labelled) RNA across 10,000 individual primary mouse fibroblasts to more broadly...
Here, we sought to directly investigate AD brain changes in cell proportion and gene expression using single-cell resolution. Methods We generated unsorted single-nuclei RNA sequencing data from brain tissue. We leveraged the tissue donated from a carrier of a Mendelian genetic mutation, PSEN1 p....
cell and single-nuclei RNA-sequencing (snRNA-seq) methods, here we show that snRNA-seq faithfully recapitulates transcriptional patterns associated with experience-driven induction of activity, including immediate early genes (IEGs) such asFos,ArcandEgr1. SnRNA-seq of mouse dentate granule cells ...
RNA sequencing of single cells (scRNA-seq) enables the global gene expression patterns of individual cells to be defined. Almost all tissues and organs include a heterogeneous mix of cell types; the heterogeneity of these cell populations can be defined through the use of scRNA-seq. scRNA-seq ...
[12] combined sNuc-Seq with microfluidic technology to introduce a single-cell nuclear RNA sequencing method with low cost, high-sensitive and high-efficiency cell classification, which is expected to be used in human cell mapping projects (Table 1). Lake et al. [13] combined single-core ...
Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid a
Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid a
cell RNA sequencing (scRNA-seq) of CD11b+myeloid cells in naïve and GL261 glioma-bearing mice that reveal distinct profiles of microglia, infiltrating monocytes/macrophages and CNS border-associated macrophages. We demonstrate an unforeseen molecular heterogeneity among myeloid cells in naïve and ...