Here, we used single-cell RNA sequencing (scRNA-seq) to decipher the composition and functions of GAMs in murine experimental GL261 gliomas grown in male and female mice. We demonstrate distinct transcriptional programs of microglia (MG), monocytes/macrophages (Mo/MΦ), and CNS BAMs. The ident...
Stimulated CD4 or CD8 T cells from PB clustered in distinct niches that also contained respiratory T cells from severely diseased patients (Fig.4a). To map this integrated data set in an unbiased and systematic manner, we applied Louvain clustering and then investigated cell type-dependent enrichme...
Molecular mechanisms underlying breast cancer lymph node metastasis remain unclear. Using single-cell sequencing, we investigated the transcriptome profile of 96,796 single cells from 15 paired samples of primary tumors and axillary lymph nodes. We identified nine cancer cell subclusters including CD44...
The single-cell RNA sequencing (scRNA-seq) is an emerging technology that provides a powerful tool for identifying cell types and cell states by analyzing the expression profile of an individual cell's transcriptome [30,31]. This technique has been successfully utilized to gain insights into the ...
Hoffman, J.A., Papas, B.N., Trotter, K.W.et al.Single-cell RNA sequencing reveals a heterogeneous response to Glucocorticoids in breast cancer cells.Commun Biol3, 126 (2020). https://doi.org/10.1038/s42003-020-0837-0 Download citation ...
title:Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis journal:cell stem cell IF: 21.46 摘要:精子发生产生成熟的雄性配子,对遗传信息的跨代的稳定传递时至关重要的。然而,人类生精过程的发育上的宏观特征目前还是未知的。我们使用了单细胞RNAseq的技术检测了...
Single-cell RNA sequencing reveals B cell–related molecular biomarkers for Alzheimer’s disease Liu-Lin Xiong, Lu-Lu Xue, Ruo-Lan Du, Rui-Ze Niu, Li Chen, Jie Chen, Qiao Hu, Ya-Xin Tan, Hui-Fang Shang, Jia Liu, Chang-Yin Yu & Ting-Hua Wang Experimental & Molecul...
Here we use highly parallel, single-cell RNA sequencing9 of malaria cultures undergoing sexual commitment to determine the transcriptional changes induced by AP2-G within this sub-population. By analysing more than 18,000 single parasite transcriptomes from a conditional AP2-G knockdown line and NF...
Zavidij, O., Haradhvala, N.J., Mouhieddine, T.H.et al.Single-cell RNA sequencing reveals compromised immune microenvironment in precursor stages of multiple myeloma.Nat Cancer1, 493–506 (2020). https://doi.org/10.1038/s43018-020-0053-3 ...
单细胞RNA测序(single-cell RNA sequencing,scRNA-seq)技术作为一项革命性工具,在单细胞水平上对转录组进行分析,已经被广泛用于多个方面的生物医学研究,包括肿瘤异质性研究、新细胞类型的鉴定、组织发育与细胞分化过程研究和基因调控网络研究等。通过scRNA-seq获得高通量的单细胞转录组数据后,利用原位组织学验证是目前流行...