基于以上问题,作者综述了目前scRNA-seq分析流程、步骤和方法(Figure 1),提出了一套目前最佳的实践分析流程,详见theislab/single-cell-tutorial. 这套分析流程在Jupyter–Ipython notebook 进行,包括R和python语言,可推荐作为一套分析模版。 Figure 1. Schematic of a typical single-cell RNA-seq analysis workflow. ...
title:Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis journal:cell stem cell IF: 21.46 摘要:精子发生产生成熟的雄性配子,对遗传信息的跨代的稳定传递时至关重要的。然而,人类生精过程的发育上的宏观特征目前还是未知的。我们使用了单细胞RNAseq的技术检测了...
RNA sequencingPROSTATE cancerEPITHELIAL cellsPROSTATETUMOR microenvironmentProstate Cancer (PCa) is one of the most common malignancies among males globally and also one of the malignant tumors with the fastest-growing incidence rate among males in China. As an epi...
单细胞RNA测序Single Cell RNA Sequencing vs. Bulk RNA Sequencing 3876 1 7:35:42 App 生肉|Single cell RNA-seq data analysis with R (2019) 单细胞RNA测序数据分析教程 2万 31 31:32 App 30分钟拿下RNA-seq原理、操作方法、分析方法和应用场景 443 -- 3:15:30 App 单细胞RNA-seq ChiPster 6599...
Different types of spiral ganglion neurons (SGNs) are essential for auditory perception by transmitting complex auditory information from hair cells (HCs) to the brain. Here, we use deep, single cell transcriptomics to study the molecular mechanisms that
CellBench package - github CellBench_data - code for the paper 现在单细胞领域的突出问题就是工具过多,但缺乏gold-standard benchmark datasets,没有一定的标准来衡量工具的好坏。 另外人们也不容易根据自己的问题来选择合适的工具,所以保险起见,现在大家都只用最有名气的那几个工具:seurat、monocle、SC3等。
所以single cell RNA-seq的技术也应运而生,这种技术首先由M Azim Surani及汤富酬创建于2009年,发表于NATURE METHOD:Tang, Fuchou, Catalin Barbacioru, Yangzhou Wang, Ellen Nordman, Clarence Lee, Nanlan Xu, Xiaohui Wang, et al. 2009. “mRNA-Seq Whole-Transcriptome Analysis of a Single Cell.” Nat...
PBDEs enhanced E2-mediated regrowth of mammary glands with terminal end bud-like structures. Analysis of mammary gland RNA revealed that PBDEs both augmented E2-facilitated gene expression and modulated immune regulation. Through single-cell RNA sequencing (scRNAseq) analysis, E2 was found to induce...
张鹍教授团队本月又在Nature Biotechnology杂志上发表了“Integrative single-cell analysis of transcriptional and epigenetic states in the human adult brain (doi: 10.1038/nbt.4038)”的文章。开发了基于冰冻组织高通量单细胞核测序方法来绘制成年人脑第二代单细胞图谱,再次引爆了单细胞研究的热点。
part/2 the rapid development of single-cell sequencing technologies provides unprecedented resolutions to study the dynamical process of cell-state transitions during development and complex disease. mathematically, the transitions can be mod...