we performed single-nuclei RNA sequencing in a Nurr1 LV-shRNA mouse model.The results revealed cellular heterogeneity in the substantia nigra and a number of activated genes,the preponderance of which encode components of the major histocompatibility Ⅱ complex.Cd74,H2-Ab1,H2-Aα,H2-Eb1,Lyz2...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
We developed a web application to provide public access to the single-nuclei transcriptomic atlas. All of the snuclRNA-seq data that we generated and processed can be queried and visualized using a custom browser that we have developed using the R Shiny framework. This browser provides an input...
Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zachary,Dalia,BarayanSarah,RehouAbdikarim,AbdullahiLauar,de Brito MonteiroMarc G.,Jeschke - 《Annals of Surgery》 被引...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which
RNAlater (CryoR), compared with Wisconsin Cold Storage Solution (CryoW) or maintenance on dry ice or at −80 °C (CryoF) until nuclei were isolated (Supplementary Fig.1b). This permitted greater flexibility in sample processing, both in terms of the physical locations at which different...
RNA sequencing (scRNA-seq) of CD11b+myeloid cells in naïve and GL261 glioma-bearing mice that reveal distinct profiles of microglia, infiltrating monocytes/macrophages and CNS border-associated macrophages. We demonstrate an unforeseen molecular heterogeneity among myeloid cells in naïve and glioma...
Nature and distribution of the single-cell RNA sequencing data The primary goal of this report is to derive a model of microglial population structure with which we can generate and test disease-related hypotheses. Figure1details the workflow and the design of this study, which included a discove...
nuclei RNA-sequencing (snRNA-seq) methods, here we show that snRNA-seq faithfully recapitulates transcriptional patterns associated with experience-driven induction of activity, including immediate early genes (IEGs) such asFos,ArcandEgr1. SnRNA-seq of mouse dentate granule cells reveals large-scale ...
healthy human liver with single-cell RNA sequencing (scRNA-seq). Although scRNA-seq is a powerful tool for describing highly heterogeneous cell populations such as those found in whole tissue2,3, it has not yet been widely applied to describe whole human organs, with only maps of isolated isl...