cell lysis, mRNA capturing, mRNA reverse transcription into cDNA, cDNA amplification, library preparation, and sequencing [19]. Herein, we will review recent research in brain cells with scRNA-seq. In the first two sections, we will discuss the advances and limitations of the methods for single...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
The selection of coronal tissue sections for snRNAseq was guided by MRI data. The maximum extent of the ischemic brain lesions was localized approximately between Bregma anterior-posterior +1.5 mm and −2 mm, in all MCAO samples, thus this region was selected for snRNAseq (Supplementary Fi...
doi:10.1002/alz.076494Fancy, Nurun NSmith, AmyTsartsalis, StergiosMuirhead, RobertChau, VickyDavey, KarenMcgarry, AislingKhozoie, CombizWillumsen, NanetZhang, XiaowenAlzheimer's & Dementia: The Journal of the Alzheimer's Association
for RNA-seq using nuclei from brain tissue or cells, which can be used to obtain global transcriptomes from neurons, glia and other cell types. Although it is described here for brain tissue, it should also be applicable to any tissue type in which dissociation of whole cells would require ...
the effect of JEV on the cellular composition and viral tropism towards distinct neuronal subtypes in the brain is still not well comprehended. To address these issues, we performed single-cell RNA sequencing (scRNA-seq) on cells isolated from the JEV-highly infected regions of mouse brain. We...
Single-nuclei transcriptome analysis of channel catfish spleen provides insight into the immunome of an aquaculture-relevant species To further examine the channel catfish immune system, we performed single-cell RNA sequencing on nuclei isolated from whole spleens, a major lymphoid ... JE Aldersey,...
Several major advantages of the method exist: first, nuclei, compared with whole cells, have the advantage of being easily isolated from complex tissues and organs, such as those in the CNS. Second, the method can be widely applied to eukaryotic species, including those of different kingdoms. ...
After data cleaning and QC (Table2), we retained and clustered 294,114 nuclei (Supplementary Dataset2), identifying 15 clusters covering major brain cell types (Fig.1b, c). These clusters were annotated based on the expression of well-known cell-type markers23(Fig.1cand Supplementary Dataset...
Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer. Nat. Commun. 8, 228 (2017). Article PubMed PubMed Central CAS Google Scholar Krishnaswami, S. R. et al. Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons. Nat. Protoc. 11...