Our method enables the transcriptome analysis of thousands of individual plant nuclei. It serves as alternative to the use of protoplast isolation, which is currently a standard methodology for plant single-cell genomics, although it can be challenging for some plant tissues. We show the ...
It serves as alternative to the use of protoplast isolation, which is currently a standard methodology for plant single-cell genomics, although it can be challenging for some plant tissues. We show the applicability of our nuclei isolation method by using different plant materials from different ...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
We performed single-cell RNA sequencing analysis of 11,612 B cells and 55,392 T cells from 12 bladder cancer patients and found naïve B cells, proliferating B cells, plasma cells, interferon-stimulated B cells and germinal center-associated B cells, and described the phenotype, gene enrichmen...
(http://ngi.pub/snuclRNA-seq) to provide full access to the research community to this resource. This is the first single-nuclei molecular atlas of AD brains carrying pathological mutations inPSEN1and related sporadic AD. We hope that this high-quality data will help elucidate and validate ...
Y. et al. Single-nucleus RNA sequencing of plant tissues using a nanowell-based system. Plant J. 108, 859–869 (2021). Article PubMed Google Scholar Neumann, M. et al. A 3D gene expression atlas of the floral meristem based on spatial reconstruction of single nucleus RNA sequencing ...
Sequencing RNA-seq Before you begin Institutional permissions All tissues from patients must be obtained with informed consent that conforms to relevant institutional and national regulations. All samples were obtained during routine surgical procedures for clinically indicated purposes. Resections were obtaine...
Nuclei were prepared from leaves of 5-week-old plants. RNA extraction and library construction For the majority of samples, RNA was isolated with the RNeasy plant mini kit (QIAGEN – including on-column DNase I treatment) according to the manufacturer’s instructions. RNA was extracted from ...
the extent to which it is useful to define subtypes of trophoblast cells and stromal cells and the relationships between cell subtypes and functions remain unclear. Single-cell RNA-seq has been a powerful tool for the identification of cell subtypes in different tissues.19,20Two studies have exam...
Nuclei of each tissue were separated by mechanical extraction. Briefly, the tissues were first thawed, infiltrated by 1× homogenization buffer (containing 30 mM CaCl2, 18 mM Mg(Ac)2, 60 mM Tris-HCl pH 7.8, 320 mM sucrose, 0.1% NP-40, 0.1 mM EDTA, and 0.2 U/µl ...