we performed single-nuclei RNA sequencing in a Nurr1 LV-shRNA mouse model.The results revealed cellular heterogeneity in the substantia nigra and a number of activated genes,the preponderance of which encode components of the major histocompatibility Ⅱ complex.Cd74,H2-Ab1,H2-Aα,H2-Eb1,Lyz2...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
We generated unsorted single-nuclei RNA sequencing data from brain tissue. We leveraged the tissue donated from a carrier of a Mendelian genetic mutation,PSEN1 p.A79V, and two family members who suffer from sporadic AD, but do not carry any autosomal mutations. We evaluated alternative alignment ...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
Single nuclear capture and sequencing were performed on the ICELL8 single-cell platform (Wafergen Biosytems). ICELL8 platform comprised of a multi-sample nano-dispenser that precisely dispensed 50 nl of the single-nuclei suspension into an ICELL8 nanowell microchip-containing 5184 wells (150...
Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zachary,Dalia,BarayanSarah,RehouAbdikarim,AbdullahiLauar,de Brito MonteiroMarc G.,Jeschke - 《Annals of Surgery》 被引...
Single-cell DNA sequencing methods are challenged by poor physical coverage, high technical error rates and low throughput. To address these issues, we developed a single-cell DNA sequencing protocol that combines flow-sorting of single nuclei, time-limited multiple-displacement amplification (MDA), ...
sequencingthetranscriptomeofacellnucleus.Nucleiareisolated fromspecimensandsortedbyFACS,cDNAlibrariesareconstructedandRNA-seqisperformed, followedbydataanalysis.Somestepsfollowpublishedmethods(Smart-seq2forcDNAsynthesis andNexteraXTbarcodedlibrarypreparation)andarenotdescribedindetailhere.Previoussingle- cellapproaches...
Complete analysis workflow for single-cell/nuclei RNA-sequencing data. workflow pipeline nextflow rnaseq single-cell nf-core single-nuclei-rna-sequencing single-nuclei Updated Jun 30, 2023 Nextflow Cadaei-Yuvxvs / snRNAseq_muscle_MND Star 0 Code Issues Pull requests Single Nuclei RNA ...
Here, we present a protocol to characterize cells expressing defined factors during direct cell reprogramming using a factor-indexing approach based on single-nuclei multiome sequencing (FI-snMultiome-seq). We describe the steps for barcoding TFs, converting human fibroblasts to pancreatic ductal-like...