Schematic diagram of a GEM-X Single Cell 3’ Gel Bead. Every Gel Bead is coated with oligos containing an Illumina TruSeq Read 1 (read 1 sequencing primer, Read 1T), 16 nt 10x Barcode, 12 nt unique molecular identifier (UMI), and 30 nt poly(dT)VN. 一旦芯片被放置在Chromium X系列仪器...
Schematic diagram of a GEM-X Single Cell 3’ Gel Bead. Every Gel Bead is coated with oligos containing an Illumina TruSeq Read 1 (read 1 sequencing primer, Read 1T), 16 nt 10x Barcode, 12 nt unique molecular identifier (UMI), and 30 nt poly(dT)VN. 一旦芯片被放置在Chromium X系列仪器...
Powerful approaches have become more accessible that allow the profiling of rare or heterogeneous populations of cells. The averaging that occurs in Conventional 'bulk' methods of sequencing does not allow the direct assessment of the fundamental biological unit—the cell—or the individual nuclei that...
The single-cell RNA sequencing (scRNA-seq) is an emerging technology that provides a powerful tool for identifying cell types and cell states by analyzing the expression profile of an individual cell's transcriptome [30,31]. This technique has been successfully utilized to gain insights into the ...
the cells are delivered at a limiting dilution. Upon dissolution of the Single Cell 3′ Gel Bead in a GEM, primers containing (i) an Illumina R1 sequence (read 1 sequencing primer), (ii) a 16 nucleotide 10x Barcode, (iii) a 10 nucleotide Unique Molecular Identifier (UMI), and (iv) ...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
Liu-Lin Xiong from the Affiliated Hospital of Zunyi Medical University, China, and coworkers used single-cell RNA sequencing to profile gene activity in individual peripheral blood mononuclear cells from people with and without Alzheimer’s disease. They discovered that people with Alzheimer’s, ...
(2019). Across five independent biological replicates, we sequenced the transcriptomes of 10 548 nuclei (see Supplemental Table 1 for sequencing details). Because some nuclear transcripts might not be spliced, we applied a "pre-mRNA" strategy to include intron-mapping reads. We obtained a median...
Single-nuclei RNA Profiling Reveals Disruption of Adipokine and Inflammatory Signaling in Adipose Tissue of Burn Patients Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zach...
Before counting UMIs, Cell Ranger attempts to correct for sequencing errors in the UMI sequences. Reads that were confidently mapped to the transcriptome are placed into groups that share the same barcode, UMI, and gene annotation. If two groups of reads have the same barcode and gene, but ...