Schematic diagram of a GEM-X Single Cell 3’ Gel Bead. Every Gel Bead is coated with oligos containing an Illumina TruSeq Read 1 (read 1 sequencing primer, Read 1T), 16 nt 10x Barcode, 12 nt unique molecular identifier (UMI), and 30 nt poly(dT)VN. 一旦芯片被放置在Chromium X系列仪器...
Powerful approaches have become more accessible that allow the profiling of rare or heterogeneous populations of cells. The averaging that occurs in Conventional 'bulk' methods of sequencing does not allow the direct assessment of the fundamental biological unit—the cell—or the individual nuclei that...
Schematic diagram of a GEM-X Single Cell 3’ Gel Bead. Every Gel Bead is coated with oligos containing an Illumina TruSeq Read 1 (read 1 sequencing primer, Read 1T), 16 nt 10x Barcode, 12 nt unique molecular identifier (UMI), and 30 nt poly(dT)VN. 一旦芯片被放置在Chromium X系列仪器...
Single-cell sequencing provides analysis of thousands of individual cells within complex environments, as opposed to traditional bulk sequencing methods that only provide a representative average of all cells. With developments in microfluidics and molecular barcoding, NGS-based technologies have increasingly...
Nuclei were processed in pools of 3–4 brains of diseased mixed with control brains, using the 10X Chromium system followed by Illumina sequencing, read alignment and processing by 10X Cellranger. Each single nucleus was matched to a specific donor using Demuxlet, confirming a 100% match by ...
the cells are delivered at a limiting dilution. Upon dissolution of the Single Cell 3′ Gel Bead in a GEM, primers containing (i) an Illumina R1 sequence (read 1 sequencing primer), (ii) a 16 nucleotide 10x Barcode, (iii) a 10 nucleotide Unique Molecular Identifier (UMI), and (iv) ...
The yield goal is approximately 10–20 million cells per mL, although we have seen that 500 cells/mL with 80% cell viability can be sufficient to perform single-cell sequencing using the 10X Genomics platform. The culturing protocol we describe is similar to dissociation protocols for immune ...
Single-cell RNA sequencing analysis identified 12 B-cell subtypes and characterized the transcriptomic heterogeneity of B cells in muscle-invasive bladder cancer (MIBC). The interferon-stimulated B-cell subtype (B-ISG15) and germinal center-associated B-cell subtypes (B-LMO2, B-STMN1) were signi...
Single-nuclei RNA Profiling Reveals Disruption of Adipokine and Inflammatory Signaling in Adipose Tissue of Burn Patients Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zach...
Liu-Lin Xiong from the Affiliated Hospital of Zunyi Medical University, China, and coworkers used single-cell RNA sequencing to profile gene activity in individual peripheral blood mononuclear cells from people with and without Alzheimer’s disease. They discovered that people with Alzheimer’s, ...