we performed single-nuclei RNA sequencing in a Nurr1 LV-shRNA mouse model.The results revealed cellular heterogeneity in the substantia nigra and a number of activated genes,the preponderance of which encode components of the major histocompatibility Ⅱ complex.Cd74,H2-Ab1,H2-Aα,H2-Eb1,Lyz2...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
We develop a method to isolate three nuclei from a pollen grain and four microspores from a tetrad for whole-genome sequencing. A high rate of aneuploidy is observed at the three-nucleus stage (6/22 pollens) rather than at the tetrad stage (1/72 microspores) in one HI line CAU5. ...
Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zachary,Dalia,BarayanSarah,RehouAbdikarim,AbdullahiLauar,de Brito MonteiroMarc G.,Jeschke - 《Annals of Surgery》 被引...
Single-cell sequencing provides analysis of thousands of individual cells within complex environments, as opposed to traditional bulk sequencing methods that only provide a representative average of all cells. With developments in microfluidics and molecular barcoding, NGS-based technologies have increasingly...
makingbrainarchivesaccessibleforRNA-seq fromindividualneurons.Themethodalsoallowsinvestigationofbiologicalfeaturesuniqueto nuclei,suchasenrichmentofcertaintranscriptsandprecursorsofsomenoncodingRNAs.By followingthisprocedure,ittakesabout4dtoconstructcDNAlibrariesthatarereadyfor sequencing. Reprintsandpermissionsinformation...
As a necessary step in gaining a deeper understanding of the regulatory mechanisms controlling plant gene expression, we report the use of single-nucleus RNA sequencing (sNucRNA-seq) and single-nucleus assay for transposase accessible chromatin sequencing (sNucATAC-seq) technologies on Arabidopsis ...
Single-cell RNA sequencing analysis identified 12 B-cell subtypes and characterized the transcriptomic heterogeneity of B cells in muscle-invasive bladder cancer (MIBC). The interferon-stimulated B-cell subtype (B-ISG15) and germinal center-associated B-cell subtypes (B-LMO2, B-STMN1) were signi...
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Single nuclear capture and sequencing were performed on the ICELL8 single-cell platform (Wafergen Biosytems). ICELL8 platform comprised of a multi-sample nano-dispenser that precisely dispensed 50 nl of the single-nuclei suspension into an ICELL8 nanowell microchip-containing 5184 wells (150...