we performed single-nuclei RNA sequencing in a Nurr1 LV-shRNA mouse model.The results revealed cellular heterogeneity in the substantia nigra and a number of activated genes,the preponderance of which encode components of the major histocompatibility Ⅱ complex.Cd74,H2-Ab1,H2-Aα,H2-Eb1,Lyz2...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
which grouped all nuclei into 6 non-neuronal and 23 neuronal (12 glutamatergic, 1 cholinergic, 10 GABAergic) major cell clusters (Fig.1b), using well-established marker genes (Fig.1c–e). We identified three neuroglia clusters, specifically one immature and one myelinating/mature oligodendrocyt...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
(<half the total entropy possible) were later identified as either having few nuclei or being enriched for carriers of genetic factors (i.e., ADAD,MS4A, orTREM2). Neuronal cell states were then categorized as excitatory (EN) or inhibitory (IN; See Methods). We capture an average of ...
Single-Nuclei RNA Sequencing Assessment of the Hepatic Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Cell Mol Gastroenterol Hepatol. 2021;11(1):147–59. Article CAS PubMed Google Scholar Hou X, Yang Y, Li P, Zeng Z, Hu W, Zhe R, et al. Integrating Spatial Transcriptomics and ...
Single-cell sequencing provides analysis of thousands of individual cells within complex environments, as opposed to traditional bulk sequencing methods that only provide a representative average of all cells. With developments in microfluidics and molecular barcoding, NGS-based technologies have increasingly...
Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zachary,Dalia,BarayanSarah,RehouAbdikarim,AbdullahiLauar,de Brito MonteiroMarc G.,Jeschke - 《Annals of Surgery》 被引...
Nuclei have several advantages over using cells: (1) nuclei can be stained with DAPI and gated to avoid collecting cells that are degraded, apoptotic, or replicating; 2) nuclei can be deposited more accurately to avoid sequencing multiple cells; and (3) nuclei can be isolated from archival ...
Single-cell RNA sequencing JEV: Japanese encephalitis virus CNS: Central nervous system DMEM: Dulbecco’s modified Eagle’s medium dpi: Days post infection IHC: Immunohistochemistry FISH: Fluorescence in situ hybridization UMI: Unique molecular identifier ...