We develop a method to isolate three nuclei from a pollen grain and four microspores from a tetrad for whole-genome sequencing. A high rate of aneuploidy is observed at the three-nucleus stage (6/22 pollens) rather than at the tetrad stage (1/72 microspores) in one HI line CAU5. ...
After the cDNA amplification, the cDNA library is constructed. cDNA libraries must be compatible with the sequencing platform. Nextera XT is a widely used library preparation kit. Libraries are generally sequenced by Illumina platforms, such as HiSeq, MiSeq and NextSeq. Single-cell RNA-sequencing ...
lipid metabolism, and neuroimmune modulation. However, the cell-specific effect of variants in these genes is not fully understood. Here, we perform single-nucleus RNA-sequencing (snRNA-seq) on nearly 300,000 nuclei from the parietal cortex of AD autosomal dominant (APPandPSEN1...
which grouped all nuclei into 6 non-neuronal and 23 neuronal (12 glutamatergic, 1 cholinergic, 10 GABAergic) major cell clusters (Fig.1b), using well-established marker genes (Fig.1c–e). We identified three neuroglia clusters, specifically one immature and one myelinating/mature oligodendrocyt...
Single-cell RNA sequencing JEV: Japanese encephalitis virus CNS: Central nervous system DMEM: Dulbecco’s modified Eagle’s medium dpi: Days post infection IHC: Immunohistochemistry FISH: Fluorescence in situ hybridization UMI: Unique molecular identifier ...
To characterize the atlas of porcine spermatogenesis, we profiled the transcriptomes of ~ 16,966 testicular cells from a 150-day-old pig testis through single-cell RNA-sequencing (scRNA-seq). The scRNA-seq analysis identified spermatogonia, spermatocytes, spermatids and three somatic cell types ...
1 mouse brain using the Chromium Nuclei Isolation Kit and enriched to remove debris. Nuclei were loaded separately onto a Next GEM Chip G (5,000 nuclei) and a GEM-X 3' chip (5,000 nuclei) and were processed on the Chromium X followed by library preparation, sequencing, and data ...
1 mouse brain using the Chromium Nuclei Isolation Kit and enriched to remove debris. Nuclei were loaded separately onto a Next GEM Chip G (5,000 nuclei) and a GEM-X 3' chip (5,000 nuclei) and were processed on the Chromium X followed by library preparation, sequencing, and data ...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
Single nuclear capture and sequencing were performed on the ICELL8 single-cell platform (Wafergen Biosytems). ICELL8 platform comprised of a multi-sample nano-dispenser that precisely dispensed 50 nl of the single-nuclei suspension into an ICELL8 nanowell microchip-containing 5184 wells (150...