为了验证鉴定小鼠interneurons的Dlx1/2调控网络,作者分析了人脑的sNuc-Seq(Single nuclei RNA-Seq)数据集。 在人类脑sNuc-Seq数据集上,SCENIC也鉴定出由DLX1/2强烈驱动的interneurons细胞群,该群具有与小鼠相同的motif,并且识别出一组保守的靶标,包括DLX1本身。 接下来,作者将这种跨物种分析扩展到其他细胞类型。与基...
we performed single-nuclei RNA sequencing in a Nurr1 LV-shRNA mouse model.The results revealed cellular heterogeneity in the substantia nigra and a number of activated genes,the preponderance of which encode components of the major histocompatibility Ⅱ complex.Cd74,H2-Ab1,H2-Aα,H2-Eb1,Lyz2...
采用 DropSeq,DroNc-seq和10xChromium平台(平台差异详见Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems)分别进行单细胞和单细胞核转录组分析,比较不同的样品类型对单细胞结果产生的影响。
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with di...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
(snRNA-seq) on nearly 300,000 nuclei from the parietal cortex of AD autosomal dominant (APPandPSEN1) and risk-modifying variant (APOE, TREM2andMS4A) carriers. Within individual cell types, we capture genes commonly dysregulated across variant groups. However, specific transcriptional states are ...
UsingsinglenucleiforRNA-seqtocapturethetranscriptomeof postmortemneurons SugunaRaniKrishnaswami 1,9 ,RashelVGrindberg 2,9 ,MarkNovotny 1 ,PratapVenepally 3 , BenjaminLacar 4 ,KunalBhutani 1 ,SaraBLinker 4 ,SonPham 4 ,JenniferAErwin 4 ,JeremyA ...
(2015) tended toward enrichment in nuclei but was not significant (NES = 0.86, padj = 8.7e-01), which is to be expected as scRNA-seq captures both nuclear and cytoplasmic RNA. RNA of genes coding for ribosomal proteins was also depleted in nuclei (NES = −2.37, padj = 3.6e-05), ...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha