We present STARsolo, a comprehensive turnkey solution for quantifying gene expression in single-cell/nucleus RNA-seq data, built into RNA-seq aligner STAR. Using simulated data that closely resembles realistic scRNA-seq, we demonstrate that STARsolo is highly accurate and significantly outperforms ...
Alzheimer’s Disease (AD) pathology has been increasingly explored through single-cell and single-nucleus RNA-sequencing (scRNA-seq & snRNA-seq) and spatial transcriptomics (ST). However, the surge in data demands a comprehensive, user-friendly repos
Systematic comparison of single-cell and single-nucleus RNA-sequencing methods Article 06 April 2020 Main Introduction: single-cell sequencing analysis Recently, single-cell sequencing technologies have been rapidly developed for observing the multilayered status of single cells. Single-cell sequencing ha...
Single cell RNA-sequencing (scRNASeq) has advanced our understanding of lung biology, but utility is limited by the need for fresh samples, loss of cell types by death or inadequate dissociation, and transcriptional stress responses induced during tissue digestion. Single nucleus RNASeq (snRNASeq)...
英文题目:Single-nucleus profiling of human dilated and hypertrophic cardiomyopathy 中文题目:人扩张性和肥厚性心肌病的单核分析 发表时间:2022-6-2 发表杂志:Nature 影响因子:69.8022 DOI:10.1038/s41569-022-00753-2摘要:心力衰竭包括一组异质性的临床特征,这些特征集中于心脏收缩功能受损上,并提出了一个日益增长...
published single-cell/single-nucleus RNA-seq (scRNA-seq/snRNA-seq) and sci-ATAC-seq datasets from mouse lung tissue (Additional file1: Fig. S6a-d; [3,30]), mouse liver tissue (Additional file1: Fig. S7a-d; [3,31]), and human lung tissue (Additional file1: Fig. S8; [32,33,...
Elucidation of immune populations with single-cell RNA-seq has greatly benefited the field of immunology by deepening the characterization of immune heterogeneity and leading to the discovery of new subtypes. However, single-cell methods inherently suffe
Single cell mRNA sequencing technologies have transformed our understanding of cellular heterogeneity and identity. For sensitive discovery or clinical marker estimation where high transcript capture per cell is needed only plate-based techniques current
RNA-seq alevin-fry - [Rust] - 🐟 Rapid, accurate and memory-frugal preprocessing of single-cell and single-nucleus RNA-seq data. anchor - [Python] - ⚓ Find bimodal, unimodal, and multimodal features in your data ascend - [R] - ascend is an R package comprised of fast, streamlined...
Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but archived human brain samples still pose a challenge to current high-throughput platforms. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting diluti...