1 Introduction to single-cell RNA-Seq and Seurat _ Bioinformatics for beginners 05:50 2 Reading single-cell data in R_ H5AD, loom, MEX, AnnData formats _ Bioinformati 16:46 3 How to analyze single-cell RNA-Seq data in R _ Detailed Seurat Workflow Tutori 36:19 4 Integrate single-cell...
第二课Introduction to Single Cell RNA Sequencing ##<IntroductiontoSingleCellRNASequencing>## 目录 ##1Commonapplications of single cell RNA sequencing.##2Overviewof single cell RNA sequencing platforms.##3ModifiedscRNA-seq workflows ##4Samplepreparation and experimental design.##5Effectsof sample prep...
可以发现新的细胞类型或者细胞状态(这里面往往蕴含着未知的生物学状态或者过程) 识别细胞发育轨迹或者病变细胞发生发展进程。 数据分析的核心是什么? QC 降维 聚类(我才是核心) 差异分析 拟时分析 富集分析 最好用的工具是什么? cellranger Seurat monocle 哪一门语言是我必须会的? R语言 畅享全文阅读体验...
single‐cell RNA‐seqQuantitative analysis of singleヽell RNA sequencing (RNA﹕eq) is crucial for discovering the heterogeneity of cell populations and understanding the molecular mechanisms in different cells. In this unit we present a bioinformatics workflow for analyzing singleヽell RNA﹕eq data ...
The single-cell RNA sequencing method can be applied to various research interests. In organ development, seemingly histologically identical tissues will eventually differentiate in various directions forming specific cell types with unique functions. Through scRNA-seq, understanding of unique gene expression...
1. Introduction to Single-cell RNA-Seq Wally the Welsh Corgi 2. Connecting & Computer Preliminaries Make sure your workshop provided computer is connected to the “Broad” or “Broad Internal” wireless network. Please do not connect your personal items (laptop, phone, etc.) to these wireless...
^Kimmel, Jacob C., et al. "Murine single-cell RNA-seq reveals cell-identity-and tissue-...
of mRNA (up to ~ 20,000 genes) from a single cell, known as the transcriptome [1]. This triggered an explosion in efforts to study heterogeneity and dynamic change in eukaryotic cells using single-cell transcriptomics, more commonly referred to as single-cell RNA-seq (scRNA-seq) [2]....
Single cell RNA sequencing, an e-book generated by the Bioinformatics team at NYU for mastering NGS analysis, including scRNA-seq analysis. Summary The above is not a comprehensive list. The landscape of single cell data analysis is evolving to incorporate new features. New and exciting tools, ...
Introduction to scRNA-seq integration Compiled: August 30, 2021 Source: vignettes/integration_introduction.Rmd The joint analysis of two or more single-cell datasets poses unique challenges. In particular, identifying cell populations that are present across multiple datasets can be problematic under sta...